other <div style="text-align: justify;"><br> <br> <span style="font-family:Times New Roman;"><span style="font-size:14px;"><span style="unicode-bidi:embed"><span style="line-height:115%"><b><span style="line-height:115%"><span style="color:black">Introduction</span></span></b><span style="line-height:115%"><span style="color:black">: Colorectal cancers have been considered devastating pathological conditions with high prevalence rates in developing and developed countries. Finding effective oncostatic compounds with fewer side effects is central to the debate. Phytocompounds with varied biological effects have been used to prevent and treat different tumors. In the present study, we investigated the tumoricidal effects of resveratrol on human colorectal HT-29 cells. </span></span></span></span><br> <span style="unicode-bidi:embed"><span style="line-height:115%"><b><span style="line-height:115%"><span style="color:black">Methods and Materials: </span></span></b><span style="line-height:115%"><span style="color:black">Cells were treated with different concentrations of resveratrol (25, 50, and 100 &micro;M) for 48 hours. The survival rate, expression, and protein levels of angiopoietin (Ang)-1 and -2 were monitored using MTT, real-time PCR, and Western blotting. </span></span></span></span><br> <span style="unicode-bidi:embed"><span style="line-height:115%"><b><span style="line-height:115%"><span style="color:black">Result:</span></span></b><span style="line-height:115%"><span style="color:black"> Data indicated the reduction of survival rate in HT-29 cells in a dose-dependent manner after being exposed to 25, 50, and 100 &micro;M of resveratrol compared to the control group (<i>p</i> = 0.05). Based on real-time PCR and Western blot analyses, increasing doses of resveratrol can reduce the expression and protein levels of Ang-1 and -2 in a dose-dependent manner (<i>p</i> = 0.05).</span></span></span></span><br> <b><span style="line-height:115%"><span style="color:black">Conclusion and Discussion:</span></span></b><span style="line-height:115%"><span style="color:black"> Resveratrol can reduce the angiogenesis properties of human colorectal HT-29 cells by suppressing Ang-1 and -2 in vitro conditions.</span></span><br> <br> <img alt="" src="./files/site1/images/888.jpg" style="width: 1000px; height: 36px;" ></span></span></div> Angiogenesis, Colorectal neoplasms, Humans, Resveratrol 222 222 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-4696-229&slc_lang=other&sid=1 Fereshteh Vaziri Nezamdoust No Masoumeh Gahramanpoor Yes Yaghub Pazhang No Reza Rahbarghazi No