Iranian Biomedical Journal

en Antioxidant Potentials, Protease Inhibitory, and Cytotoxic Activities of Various Isolated Extracts from Salvia aegyptiaca Related Fields Related Fields مقاله کامل Full Length/Original Article <div style="text-align: justify;">Background: Natural compounds can regulate the growth and progression of cancer cells with low toxicity to normal cell; therefore, these compounds are unique targets for cancer treatment. Recently, extracts from Salvia species have shown promising antiproliferative potential. This study aimed to isolate and characterize bioactive compounds from Salvia aegyptiaca and evaluate their antioxidant, cytotoxic, and protease-inhibitory activities. Methods: In this study, various extracts of S. aegyptiaca were prepared, and several compounds, including ursolic acid, oleanolic acid, luteolin-7-O-glucoside, quercetin-3-O-rutoside, and rosmarinic acid, were isolated and characterized using different spectroscopic methods. Finally, the antioxidant activity, protease inhibitory activity, and cytotoxicity of the crude extract, multiple fractions, and isolated compounds were examined. Results: According to the results obtained, rosmarinic acid demonstrated the highest antioxidant performance, as indicated by the following assays: DPPH (IC50: 28.39 ± 0.75 µg/mL), ABTS (39.52 ± 0.72 µg/mL), FRAP (31.87 ± 0.67 µg/mL), NO scavenging (71.44 ± 1.04 µg/mL), and ORAC values (0.6 TE/mg). Furthermore, both cynaroside and rosmarinic acid exhibited the most potent antiproliferative effects against the Hep G2 cell line, with IC50  value of 34.4 ± 2.34 and 47.84 ± 5.87 µg/mL, respectively. The EtOAc fraction and rosmarinic acid also showed higher protease inhibitory activity, with IC50 of 17.6 ± 0.10 and 17.0 ± 0.30 µg/mL, respectively, as compared to other compounds. Conclusion: Our findings suggest that the identified compounds may be responsible for the antiproliferative effects of S. aegyptiaca. Overall, S. aegyptiaca could serve as a valuable natural antioxidant and anticancer agent in both pharmaceutical and food industries.</div> Salvia aegyptiaca, Antioxidant activity, Protease inhibitory, Cytotoxicity 57 67 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-6046-1&slc_lang=en&sid=1 Ali Hosseini saiz@yahoo.com 0000-0002-9714-3221 No Department of Pharmacognosy, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran Mahmoodreza Moein mrezamoein@yahoo.com 0000-0002-3355-4654 Yes Department of Pharmacognosy, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran Zahra Sabahi* sabahiz@yahoo.com 0000-0002-6760-1009 No Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Soheila Moein saiz@gmail.com 0000-0001-8465-3604 No Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Salar Hafez Ghoran Ahmadi@yahoo.com 0000-0001-5495-5556 No H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Pakistan Moslem Naderian Farzaneh22@yahoo.com 0000-0003-2971-9978 No Department of Pharmacognosy, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran Zahra Zebarjad FarzanehYavari@yahoo.com 0000-0001-9710-6458 No Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran