en Molecular Microbiology Molecular Microbiology مقاله مروری Review Article <div style="text-align: justify;"><span style="font-family:Times New Roman;"><span style="font-size:10.5pt"><span style="line-height:115%"><span style="color:black">Cutaneous leishmaniasis (CL) is a common form of leishmaniasis in underdeveloped countries. Although CL tends to be self-limiting, it can cause significant scars and may progress to more severe manifestations. Additionally, <i>Leishmania</i> species vary in susceptibility to the available treatments. The selection of treatment and clinical outcome of CL depend on the accurate determination of the <i>Leishmania</i> species. This mini-review aims to provide an overview of the molecular diagnosis techniques such as PCR-based assays, nucleic acid sequence-based amplification, and loop-mediated isothermal amplification utilized in the identification of <i>Leishmania</i> species in Iran. </span></span></span></span></div> Humans, Iran, Leishmaniasis, Molecular diagnostic techniques 148 155 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-5863-1&slc_lang=en&sid=1 Seyed Ebrahim Eskandari crtsdl_eskandari@yahoo.com 0009-0004-1756-6577 Yes Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, Iran Mojtaba Memariani memaryani@gmail.com 0000-0002-4964-3117 No Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran Hamed Memariani h.memariani@gmail.com 0000-0003-1026-140X No Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran Mehdi Mohebali mohebali@tums.ac.ir 0000-0002-4164-9514 No Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Ali Khamesipour ali.khamesipour@gmail.com 0000-0003-3165-9337 No Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, Iran