en Related Fields Related Fields سیتماتیک ریویو Systematic Review <div style="text-align: justify;"><span style="font-family:Times New Roman;"><span style="font-size:10.5pt"><span style="line-height:115%"><span style="color:black">Feasible diagnostic assays are required to detect new tuberculosis (TB) cases and monitor treatment. This study aimed to evaluate evidence on </span></span></span><span style="font-size:10.5pt"><span style="line-height:115%">t</span></span><span lang="IN" style="font-size:10.5pt"><span style="line-height:115%">ryptophan</span></span><span style="font-size:10.5pt"><span style="line-height:115%"><span style="color:black"> (Trp) and its metabolites as proposed biomarkers for TB. Through specific keyword searches, we identified 170 relevant literature sources and included seven publications (from 2013 to 2023). The biomarker used in these studies were indoleamine 2, 3-dioxygenase (IDO) activity, IDO-1 gene expression, and plasma IDO protein, measured using ELISA, </span></span></span><span style="font-size:10.5pt"><span style="line-height:115%">liquid chromatography-mass spectrometry<span style="color:black">, </span></span></span><span style="font-size:10.5pt"><span style="line-height:115%">ultraperformance liquid chromatography mass spectrometry</span></span><span style="font-size:10.5pt"><span style="line-height:115%"><span style="color:black">, and transcriptional profiling. The studies encompassed a pediatric case-control and six studies involving adults, pregnant women with TB-HIV, and individuals with </span></span></span><span style="font-size:10.5pt"><span style="line-height:115%"><span style="color:black">multidrug-resistant </span></span></span><span style="font-size:10.5pt"><span style="line-height:115%"><span style="color:black">tuberculosis, active TB, and latent TB. The assessment of IDO activity and IDO protein level demonstrated promising performance in distinguishing active TB from controls and in evaluating treatment failure and recurrent cases to controls. </span></span></span><span lang="IN" style="font-size:10.5pt"><span style="line-height:115%">Trp</span></span> <span style="font-size:10.5pt"><span style="line-height:115%"><span style="color:black">and its metabolites fulfilled&nbsp; nearly all of target product profile criteria for detecting active TB. This study highlights the potential of utilizing host </span></span></span><span lang="IN" style="font-size:10.5pt"><span style="line-height:115%">Trp</span></span> <span style="font-size:10.5pt"><span style="line-height:115%"><span style="color:black">and its metabolites as non-sputum-based biomarker for TB infection.</span></span></span> </span></div> Biomarkers, Tryptophan, Tuberculosis 140 147 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-5774-1&slc_lang=en&sid=1 Novi Maulina novimaulina@usk.ac.id 0000-0003-1218-9536 No Doctorate Student of Doctoral Program in Medical Sciences, Faculty of Medicine, Universitas Syiah Kuala, Banda Aceh, 23116, Indonesia Zinatul Hayati hayatikarmil@usk.ac.id 0000-0003-3775-0422 Yes Microbiology Department, Faculty of Medicine, Universitas Syiah Kuala, Banda Aceh, 23116, Indonesia Kartini Hasballah kartini.hasballah@usk.ac.id 0000-0002-1792-3990 No Pharmacology Department, Faculty of Medicine, Universitas Syiah Kuala, Banda Aceh, 23116, Indonesia Zulkarnain Zulkarnain zulkarnain_md@usk.ac.id 0000-0002-5395-1892 No Physiology Department, Faculty of Medicine, Universitas Syiah Kuala, Banda Aceh, 23116, Indonesia