en Molecular Genetics & Genomics Molecular Genetics & Genomics مقاله کوتاه Short Communication <div style="text-align: justify;"><span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:10.5pt"><span style="color:black">Background</span></span></b><span style="font-size:10.5pt"><span style="color:black">: </span></span><span style="font-size:10.5pt">E</span><span style="font-size:10.5pt"><span style="color:black">xosomal RNAs (ExoRNAs) offer valuable insights into their cellular origin. ExoRNA studies were faced with challenges in obtaining sufficient amounts of high-quality RNA. Herein, we aimed to compare three traditional exosome isolation methods to introduce an appropriate strategy to extract RNA from cancer-derived exosomes for further RNA analysis.<span style="background:lime"></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:10.5pt"><span style="color:black">Methods</span></span></b><span style="font-size:10.5pt"><span style="color:black">: Exosomes were isolated through ultracentrifugation, precipitation kit, and size exclusion column chromatography, and then characterized by </span></span><span style="font-size:10.5pt"><span style="color:black">dynamic light scattering</span></span><span style="font-size:10.5pt"><span style="color:black"> and </span></span><span style="font-size:10.5pt"><span style="color:black">transmission electron microscopy, followed by extracting total RNA. The quality and quantity of the extracted RNAs were assessed by a NanoDrop and 2.5% agarose gel electrophoresis.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:10.5pt"><span style="color:black">Results: </span></span></b><span style="font-size:10.5pt"><span style="color:black">Extracted exosomes<b> </b>displayed a similar range of size and morphology. We found that </span></span><span style="font-size:10.5pt"><span style="color:black">polyethylene glycol-precipitation method resulted in a higher RNA yield with a 260/280 ratio of 1.9. The obtained exoRNA appeared as a smear in the agarose gel, indicative of small exoRNAs.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:10.5pt"><span style="color:black">Conclusion: </span></span></b><span style="font-size:10.5pt"><span style="color:black">We provide researchers a suitable approach to isolate exosomes based on yield and purity of exoRNA.</span></span><span style="font-size:10.5pt"></span></span></span></span><br> &nbsp;</div> Exosomes, Extracellular vesicles, MicroRNA 132 139 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-5697-1&slc_lang=en&sid=1 Abdulwahab Teflischi Gharavi abdulwahabteflischie@khu.ac.ir 0000-0002-7153-4312 No Department of Cell and Molecular Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran Azadeh Niknejad niknejad@khu.ac.ir 0000-0001-7808-488X No Department of Cell and Molecular Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran Saeed Irian Irian@khu.ac.ir 0000-0002-4190-2380 No Department of Cell and Molecular Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran Amirabbas Rahimi amirabbas_rahimi@yahoo.com 0000-0002-1982-8081 No Molecular Medicine Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran Mona Salimi salimi_mona@yahoo.com 0000-0002-3997-7009 Yes Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran