en Cancer Biology Cancer Biology مقاله کامل Full Length/Original Article <div style="text-align: justify;"><span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:10.5pt"><span style="color:black">Background</span></span></b><span style="font-size:10.5pt"><span style="color:black">: The 17beta-hydroxysteroid dehydrogenase type 1 (HSD17B) family has been implicated in the prognosis and treatment prediction of various malignancies; however, its association with bladder cancer (BLCA) remains unclear. This study aimed to evaluate the potential of <em>HSD17B1</em>, as a prognostic biomarker, for the survival of patients with BLCA and to determine its effectiveness as a supplemental biomarker for BLCA.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:10.5pt"><span style="color:black">Methods:</span></span></b><span style="font-size:10.5pt"> <span style="color:black">A series of bioinformatics techniques were applied to investigate the expression of <i>HSD17B1</i> in different types of cancer and its potential association with the prognosis of BLCA patients using diverse databases. The UALCAN, Human Protein Atlas, cBioPortal, Metascape, GEPIA, MethSurv, and TIMER were employed to analyze expression differences, mutation status, enrichment analysis, overall survival, methylation, and immune-infiltrating cells. The real-time reverse transcription-PCR </span><span style="color:black">(qRT-PCR) was implemented to detect the messenger ribonucleic acid (mRNA) expression levels of <i>HSD17B1</i> in vitro. </span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:10.5pt"><span style="color:black">Results: </span></span></b><span style="font-size:10.5pt"><span style="color:black">Elevated mRNA and protein levels of <i>HSD17B1</i>, surpassing normal levels, were observed in BLCA samples. In addition, the BLCA patients with higher mRNA expression level of <i>HSD17B1</i> significantly reduced the overall survival. </span>Also, several immune infiltrating cells, including mast cell resting CIBERSORT-ABS, have been identified as tumor-associated biomarker genes, with the potential to significantly influence the immunological environment. <span style="color:black">Finally, qRT-PCR analysis revealed a significant upregulation of <i>HSD17B1</i> mRNA expression level in the cancer cells compared to the human 293T cells, which was consistent with the bioinformatics data.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:10.5pt"><span style="color:black">Conclusion</span></span></b><span style="font-size:10.5pt"><span style="color:black">: There is a strong correlation between the elevated <i>HSD17B1</i> expression and positive prognosis in patients with BLCA. Therefore, <i>HSD17B1</i> can be used as a prognostic biomarker in these patients.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:107%"><span style="font-size:10.5pt"><span style="line-height:107%"></span></span></span></span></span><br> &nbsp;</div> Biomarkers, HSD17B1, Survival 120 131 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-5610-1&slc_lang=en&sid=1 Aida Albadawy ismailaid49@gmail.com 0009-0006-6101-0677 No State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China Mohammed Alqudaimi malqudaimi199@gmail.com 0009-0005-0904-4776 No State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China Hanyue Cui yywyyxmdzz@163.com 0009-0001-5379-708X No State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China Xianghui Yan 1587230498@qq.com 0009-0003-9274-6837 No State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China Jing Sun sunj@nwipb.cas.cn 0000-0002-8255-3295 No Qinghai Key Laboratory of Qinghai-Tibet Plateau Biological Resources, Northwest Institute of Plateau Biology, The Chinese Academy of Sciences, Xining 810001, China Ping Shi ship@ecust.edu.cn 0000-0002-6904-2802 Yes State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China