Iranian Biomedical Journal

en Role of Native Probiotic Lactobacillus Species via TGF-β Signaling Pathway Modulation in CRC Molecular Microbiology Molecular Microbiology مقاله کامل Full Length/Original Article <div style="text-align: justify;">Background: Colon microbiome composition in colorectal cancer (CRC) patients undergoes remarkable changes. The present study was designed to assess the impact of Lactobacillus mixture on the regulating the CRC by influencing the transforming growth factor beta (TGF-β) signaling pathway in both in vitro (HT-29 cancer cells) and in vivo (BALB/c mice) models. Methods: In this study, the antiproliferative effect of a native potential probiotic Lactobacillus mixture on HT-29 cancer cells was evaluated using the MTT assay method. Also, qRT-PCR was performed to assess the RNA expression level of genes associated with the TGF-β signaling pathway at three levels: receptor, regulatory, and inhibitory SMADs. Finally, the in vivo assays were investigated by three groups of mice: a naive group (PBS), a disease group (azoxymethane [AOM]/ dextran sulfate sodium [DSS] + PBS), and a treatment group (AOM/DSS + Lactobacillus mixture in PBS). Results: The MTT results showed a significant decrease in proliferation of HT-29 cancer cells after 120 h of treatment. Furthermore, qRT-PCR demonstrated the downregulation of the smad2/3 gene expression in HT-29-treated cells and also reduction in the level of smad4 gene expression. In addition, in the mouse model, the tgf-βR1 gene was downregulated in the group treated with AOM/DSS/Lactobacillus, but not the AOM/DSS group. A downregulation of smad4 gene expression was also observed in in vivo models. Conclusion: The obtained results suggest that our novel probiotic Lactobacillus mixture could have a positive impact on the inhibition of the CRC progression by downregulating the TGF-β signaling pathway.</div> Colorectal cancer, Lactobacillus, Probiotics, TGF-β 168 178 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-4792-2&slc_lang=en&sid=1 Amin Sepehr aminsepehr20@gmail.com 0000-0001-6666-2005 No Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran Shadi Aghamohammad No Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran Roya Ghanavati 0000-0002-3851-0001 No Behbahan Faculty of Medical Sciences, Behbahan, Iran Malihe Talebi 0000-0002-9111-8121 No Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran Mohammad Reza Pourshafie* 0000-0002-6226-7064 No Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran Mahdi Rohani 0000-0001-8956-5072 Yes Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran