en Cancer Biology Cancer Biology مقاله کامل Full Length/Original Article <div style="text-align: justify;"><span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:115%"><span calibri=""><b><span new="" roman="" times=""><span style="color:black">Background:</span></span></b><span new="" roman="" times="">&nbsp;<span style="color:black">The surface properties of dental and orthopedic implants are directly related to their osseointegration rate. Coating and/or modifying the implant surface might reduce the time of healing. </span><span lang="EN" new="" roman="" times=""><span style="color:black">In this study, we aimed to examine the effects of a hybrid surface consisting of a brushite surface coating and cross-linked water-soluble eggshell membrane protein on the osseointegration of </span></span></span><span new="" roman="" times=""><span style="color:black">titanium</span></span><span lang="EN" new="" roman="" times=""><span style="color:black"> (Ti) screws under in vivo conditions.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:115%"><span calibri=""><b><span new="" roman="" times=""><span style="color:black">Methods:</span></span></b><span new="" roman="" times="">&nbsp; <span style="color:black">Twenty Ti alloy screws were implanted monocortically in anteromedial regions of New Zealand rabbit tibiae. Ten screws were untreated and used as controls. The remaining 10 screws were coated with calcium phosphate and following cross-linked with ostrich eggshell membrane protein. All rabbits were sacrificed six weeks after the surgery. Peri-screw tissues were evaluated by </span>micro-computed tomography (&micro;-CT), histological and histomorphometrical methods.&nbsp; </span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:115%"><span calibri=""><b><span new="" roman="" times=""><span style="color:black">Results:</span></span></b><span new="" roman="" times="">&nbsp;<span style="color:black">The &mu;-CT assessments indicated that the experimental group had significantly higher mean </span>bone surface area (BSA) and trabeculae number (TbN) than those of the control group (<i>p </i>˂ 0.05). Bone surface area (BV), trabecular separation (TbSp), trabecular thickness (TbTh), and bone mineral density (BMD) scores of the control and experimental groups were quite similar (<i>p </i>> 0.05). The vascularization score of the experimental group was significantly higher than the control group (4.29 vs. 0.92%). No sign of the graft-versus-host reaction was observed. </span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:115%"><span calibri=""><b><span new="" roman="" times=""><span style="color:black">Conclusion:</span></span></b><span new="" roman="" times="">&nbsp; <span style="color:black">Our findings reveal that coating Ti alloy implants with calcium phosphate cross-linked with ostrich eggshell membrane protein increases the osseointegration of Ti alloy screws by increasing the bone surface area, number of trabeculae and vascularization in the implant site.</span></span></span></span></span><br> &nbsp;</span></div> Bee venom, Colorectal neoplasms, Unfolded protein response, Autophagy 46 52 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-5526-1&slc_lang=en&sid=1 Mozhdeh Zamani mozhdeh.zamani63@gmail.com 0000-0001-7856-6029 No Autophagy Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Farzaneh Bozorg-Ghalati fbozorgghalati@yahoo.com 0000-0001-9073-7820 No Autophagy Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Pooneh Mokarram mokaram2@gmail.com 0000-0002-9717-0473 Yes Autophagy Research Center, Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran