Iranian Biomedical Journal
مجله بیومدیکال ایران
IBJ
Basic Sciences
http://ibj.pasteur.ac.ir
1
admin
1028-852X
2008-823X
-
10.61882/ibj
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8888
-
en
jalali
1402
6
1
gregorian
2023
9
1
27
5
online
1
fulltext
en
Formulation and Characterization of a Novel Oxidized Alginate-Gelatin-Silk Fibroin Bioink with the Aim of Skin Regeneration
Tissue Engineering and Cell Biology
Tissue Engineering and Cell Biology
مقاله کامل
Full Length/Original Article
<span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:115%"><b><span style="font-size:10.5pt"><span style="line-height:115%"><span style="color:black">Background: </span></span></span></b><span style="font-size:10.5pt"><span style="line-height:115%"><span style="color:black">In the present study, a novel bioink was suggested based on the </span></span></span><span style="font-size:9.0pt"><span style="line-height:115%"><span style="color:black">oxidized alginate</span></span></span><span style="font-size:10.5pt"><span style="line-height:115%"><span style="color:black"> (OAlg), </span></span></span><span style="font-size:9.0pt"><span style="line-height:115%"><span style="color:black">gelatin</span></span></span><span style="font-size:10.5pt"><span style="line-height:115%"><span style="color:black"> (GL), and </span></span></span><span style="font-size:9.0pt"><span style="line-height:115%"><span style="color:black">silk fibroin</span></span></span><span style="font-size:10.5pt"><span style="line-height:115%"><span style="color:black"> (SF) hydrogels. </span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:115%"><b><span style="font-size:10.5pt"><span style="line-height:115%"><span style="color:black">Methods:</span></span></span></b><span style="font-size:10.5pt"><span style="line-height:115%"><span style="color:black"> The composition of the bioink was optimized by the rheological and printability measurements, and the extrusion-based 3D bioprinting process was performed by applying the optimum OAlg-based bioink. </span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:115%"><b><span style="font-size:10.5pt"><span style="line-height:115%"><span style="color:black">Results: </span></span></span></b><span style="font-size:10.5pt"><span style="line-height:115%"><span style="color:black">The results demonstrated that the viscosity of bioink was continuously decreased by increasing the SF/GL ratio, and the bioink displayed a maximum achievable printability (92 ± 2%) at 2% (w/v) of SF and 4% (w/v) of GL. Moreover, the cellular behavior of the scaffolds investigated by MTT assay and live/dead staining confirmed the biocompatibility of the prepared bioink. </span></span></span></span></span><br>
<b><span style="font-size:10.5pt"><span style="line-height:115%"><span style="color:black">Conclusion: </span></span></span></b><span style="font-size:10.5pt"><span style="line-height:115%"><span style="color:black">The bioprinted OAlg-GL-SF scaffold could have the potential for using in skin tissue engineering applications, which needs further exploration. </span></span></span></span>
Alginate, Bioprinting, Fibroin, Gelatin
280
293
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-5512-1&slc_lang=en&sid=1
Khadijeh
Sanaei
ksanaei2004@gmail.com