en Related Fields Related Fields مقاله کامل Full Length/Original Article <div style="text-align: justify;"><span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:10.5pt"><span style="color:black">Background:</span></span></b><span style="font-size:10.5pt"><span style="color:black"> Traumatic brain injury or TBI can underlie epilepsy. </span></span><span style="font-size:10.5pt"><span style="color:black">Prevention of PTE has been of great interest to scientists. Given the antiepileptic, antioxidant and anti-inflammatory activities of curcumin, we examined whether this compound can affect epileptogenesis in rats after TBI.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:10.5pt"><span style="color:black">Methods:</span></span></b><span style="font-size:10.5pt"><span style="color:black"> Curcumin was injected once a day for two weeks. TBI was induced in the temporal cortex of anesthetized rats using a </span></span><span style="font-size:9.0pt"><span style="color:black">controlled cortical impact</span></span><span style="font-size:10.5pt"><span style="color:black"> device. One day after TBI, </span></span><span style="font-size:9.0pt"><span style="color:black">pentylenetetrazole</span></span><span style="font-size:10.5pt"><span style="color:black"> (PTZ), 35 mg/kg, was injected i.p. every other day until manifestation of generalized seizures. The number of PTZ injections was then recorded. Moreover, the extent of cortical and hippocampal IL-1&beta; and </span></span><span style="font-size:9.0pt"><span style="color:black">glial fibrillary acidic protein</span></span><span style="font-size:10.5pt"><span style="color:black"> (GFAP) expression in the epileptic rats were measured by Western blot analysis. </span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:10.5pt"><span style="color:black">Results: </span></span></b><span style="font-size:10.5pt"><span style="color:black">Curcumin 50 and 150 mg/kg prevented the development of kindling, whereas TBI accelerated the rate of kindling. Curcumin 20 mg/kg prohibited kindling facilitation by TBI, and reduced the expression of IL-1&beta; and GFAP induced by TBI. </span></span></span></span><br> <b><span style="font-size:10.5pt"><span style="line-height:115%"><span style="color:black">Conclusion: </span></span></span></b><span style="font-size:10.5pt"><span style="line-height:115%"><span style="color:black">Curcumin can stop the acceleration of epileptogenesis after TBI in rats. Inhibiting hippocampal and cortical overexpression of IL-1&beta; and GFAP seems to be involved in this activity.</span></span></span> </span></div> Glial fibrillary acidic protein, IL-1β, Post-traumatic epilepsy, Traumatic brain injury 113 119 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-5498-1&slc_lang=en&sid=1 Hanieh Jahi hany_jahi@yahoo.com 0009-0006-8666-049X No Department of Physiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran Mansoureh Eslami eslami342@yahoo.com 0000-0002-4967-4829 Yes Department of Basic Sciences, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran Mohammad Sayyah* sayyahm2@yahoo.com 0000-0003-0603-2444 No Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran Fariba Karimzadeh fariba_karimzadeh@yahoo.com 0000-0002-8805-3486 No Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran Melika Alesheikh melikaalesheikh@yahoo.com 0009-0004-4225-1059 No Department of pharmacy, Tehran University of Medical Sciences, Tehran, Iran