en Tissue Engineering and Cell Biology Tissue Engineering and Cell Biology مقاله کامل Full Length/Original Article <div style="text-align: justify;"><span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:10.5pt"><span style="color:black">Background: </span></span></b><span style="font-size:10.5pt">Synthetic and natural polymer scaffolds can be used to design wound dressing for repairing the damaged skin tissue. This study investigated acute wound healing process using a decellularized skin scaffold and <span style="color:black">mouse embryo fibroblast</span> (MEF).</span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:10.5pt"><span style="color:black">Methods</span></span></b><span style="font-size:10.5pt"><span style="color:black">: </span>Mouse skin fragments were decellularized and evaluated by DNA content, toxicity, H&E staining, <span style="color:black">Raman confocal microscopy, </span>Masson&rsquo;s trichrome<span style="background:white"><span style="color:black"> staining, </span></span>SEM, and biodegradation assays. The fragments were recellularized by the MEFs, and cell attachment and penetration were studied. De- and decellularized scaffolds were used wound dressings in mouse acute wound models as two experimental groups. Using morphological and immunohistochemical assessments, wound healing was evaluated and compared among the experimental and control groups.</span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:10.5pt"><span style="color:black">Results: </span></span></b><span style="font-size:10.5pt">DNA content of the decellularized tissue significantly reduced compared to the native control group (7% vs. 100%; <i>p</i> < 0.05). <span style="color:black">extracellular matrix</span> components, e.g. collagen types I, III, and IV, elastin, and glycosaminoglycan, were well preserved in the decellularized group. The porosity and fiber arrangement in the stroma had a structure similar to normal skin tissue. A significant reduction in healing time was observed in the group treated with a decellularized scaffold. A thicker epidermis layer was observed in the recovered tissue in both experimental groups compared to the control group. Immunostaining showed a positive reaction for CD31 as an endothelial marker in both experimental groups, confirming new vascularization in these groups. </span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:10.5pt"><span style="color:black">Conclusion: </span></span></b><span style="font-size:10.5pt">Using MEFs with decellularized skin as a wound dressing increases the rate of wound healing and also the formation of new capillaries. This system could be beneficial for clinical applications in the field of tissue engineering. </span></span></span></span><br> &nbsp;</div> Decellularized extracellular matrix, Neovascularization, Skin, Wound healing 90 101 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-227-11&slc_lang=en&sid=1 Armaghan Gheytasvand a.gheytasvand@modares.ac.ir 0009-0002-0312-719X No Department of Biomaterials, Faculty of Interdisciplinary Science and Technology, Tarbiat Modares University, Tehran, Iran Hamed Bagheri hbagheri@modares.ac.ir 0000-0001-6923-4954 No Department of Biomaterials, Faculty of Interdisciplinary Science and Technology, Tarbiat Modares University, Tehran, Iran Shahram Pourbeyranvand shahrampb@modares.ac.ir 0000-0002-4708-6548 No Department of Anatomical Sciences, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran Mojdeh Salehnia mojdehsalehnia@gmail.com 0000-0001-7861-2232 Yes Department of Biomaterials, Faculty of Interdisciplinary Science and Technology, Tarbiat Modares University, Tehran, Iran