Iranian Biomedical Journal
مجله بیومدیکال ایران
IBJ
Basic Sciences
http://ibj.pasteur.ac.ir
1
admin
1028-852X
2008-823X
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10.61882/ibj
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8888
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en
jalali
1402
6
1
gregorian
2023
9
1
27
5
online
1
fulltext
en
CRISPR-Cas Technology as a Revolutionary Genome Editing tool: Mechanisms and Biomedical Applications
Medical Biotechnology
Medical Biotechnology
مقاله مروری
Review Article
<span style="font-family:Times New Roman;"><span style="font-size:10.5pt"><span style="line-height:115%">Programmable nucleases are powerful genomic tools for precise genome editing. These tools precisely recognize, remove, or change DNA at a defined site, thereby stimulating cellular DNA repair pathways that can cause mutations or accurate replacement or deletion/insertion of a sequence. The CRISPR-Cas9 system is the most potent and useful genome editing technique adapted from the immune system of certain bacteria and archaea against viruses and phages. In the past decade, this technology has made notable progress, and at present, it has largely been used in genome manipulation to make precise gene editing in plants, animals, and human cells. In this review, we aimed to explain the basic principles, mechanisms of action, and applications of this system in different areas of medicine, with an em<span style="background:white"><span style="color:#222222">phasize on the </span></span>detection and treatment of parasitic diseases. </span></span></span>
CRISPR-Cas systems, Gene editing, Medicine, Parasitology
219
246
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1166-2&slc_lang=en&sid=1
Sahar
Ebrahimi
Ebrahimi.sahar67@gmail.com