Iranian Biomedical Journal

en Evaluation of Vascular Endothelial Growth Factor Gene Expression in Recellularized Liver Tissue by Mouse Embryo Fibroblast Tissue Engineering and Cell Biology Tissue Engineering and Cell Biology مقاله کامل Full Length/Original Article Background: The aim of the present study was to evaluate alterations in the vegf gene expression as an angiogenic factor in mouse embryo fibroblasts seeded on the decellularized liver fragments. Methods: Liver tissue samples (n = 10) collected from adult male mice were randomly divided into decellularized and native control groups. Tissues were decellularized by treating with 1% Triton X-100 and 0.1% SDS for 24 hours and assessed by H&E staining and scanning electron microscopy (SEM). Then DNA content analysis and toxicity tests were performed. By centrifugation, DiI-labeled mouse embryo fibroblasts were seeded on each scaffold and cultured for one week. The recellularized scaffolds were studied by H&E staining, SEM, and laser scanning confocal microscopy (LSCM). After RNA extraction and cDNA synthesis, the expression of the vegf gene in these samples was investigated using real-time RT-PCR. Results: Our observations showed that the decellularized tissues had morphology and porous structure similar to the control group, and their DNA content significantly reduced (p < 0.05) and reached to 4.12% of the control group. The MTT test indicated no significant cellular toxicity for the decellularized scaffolds. Light microscopy, SEM, and LSCM observations confirmed the attachment and penetration of embryonic fibroblast cells on the surface and into different depths of the scaffolds. There was no statistically significant difference in terms of vegf gene expression in the cultured cells in the presence and absence of a scaffold. Conclusion: The reconstructed scaffold had no effect on vegf gene expression. Decellularized mouse liver tissue recellularized by embryonic fibroblasts could have an application in regenerative medicine. Vascular endothelial growth factor, Gene expression, Fibroblast, Liver, Decellularized ECM 340 348 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-227-10&slc_lang=en&sid=1 Motahare Homayoon Vala m-homayoonvala@modares.ac.ir 0000-0002-3557-8457 No Department of Biomaterials, Faculty of Interdisciplinary Science and Technology, Tarbiat Modares University, Tehran, Iran Hamed Bagheri hbagheri@modares.ac.ir 0000-0001-6923-4954 No Department of Biomaterials, Faculty of Interdisciplinary Science and Technology, Tarbiat Mdares University, Tehran, Iran Zinat Sargazi zinat.sargazi@modares.ac.ir 0000-0002-7017-4423 No Department of Anatomical Sciences, School of Medical Sciences, Zahedan University, Zahedan, Iran Negar Bakhtiary negarbakhtiari@modares.ac.ir 0000-0003-2771-0339 No 1Department of Biomaterials, Faculty of Interdisciplinary Science and Technology, Tarbiat Modares University, Tehran, Iran Shahram Pourbeiranvand shahrampb@modares.ac.ir 0000-0002-4708-6548 No Department of Anatomical Sciences, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran Mojdeh Salehnia salehnim@modares.ac.ir 0000-0001-7861-2232 Yes Department of Biomaterials, Faculty of Interdisciplinary Science and Technology, Tarbiat Modares University, Tehran, Iran