Iranian Biomedical Journal

en Anticancer Effect of Enterococcus faecium, Isolated from Vaginal Fluid, on Ovarian Cancer Cells Molecular Microbiology Molecular Microbiology مقاله کامل Full Length/Original Article <div style="text-align: justify;">Background: Given the association between cervicovaginal microbiota and OVC, we investigated the effect of Enterococcus faecium conditioned medium (CM) on OVC (Caov-4) cells. Methods: CM was obtained from the bacterium E. faecium isolated from the vagina of healthy women. The Caov-4 cells were treated with varying concentrations of CM that comprised co-cultured bacteria with 0.2, 0.5, 1, 1.5, and 2 OD for 12, 24, and 48 h. The apoptosis and growth of cancer cells were evaluated by 4′,6-diamidino-2-phenylindole (DAPI) staining, flow cytometry, and DNA laddering assay. Moreover, the expression of PTEN, BAX, BCL2, and AKT1 genes were analyzed using real-time PCR.  Results: The CM at a concentration of 0.5 OD from the cultured bacteria and incubation time of 48 h showed the highest negative effect on the viability of cancer cells. The CM treatment increased DNA fragmentation and also induced apoptosis in Caov-4 cells. Interestingly, CM could decrease the expression of proapoptotic genes were less, while antiapoptotic genes were more than fluorouracil in the presence of CM. Conclusion: CM of human-derived E. faecium could have an anticancer effect on OVC cells in a concentration- and time-dependent manner. This study demonstrated that E. faecium secretes anticancer substances into the CM, which could directly affect the viability and apoptosis of cancer cells.</div> Apoptosis, Enterococcus faecium, Ovarian neoplasms 205 213 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-5172-1&slc_lang=en&sid=1 Soraya Pourmollaei 0000-0002-6939-5253 No Department of Midwifery, Faculty of Nursing and Midwifery, Tabriz University of Medical Sciences, Tabriz, Iran Azizeh Farshbaf-Khalili 0000-0001-5754-4613 No Aging Research Institute, Physical Medicine, and Rehabilitation Research Centre, Tabriz University of Medical Sciences, Tabriz, Iran Abolfazl Barzegari 0000-0002-8429-3618 No Research Center of Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran Sepideh Bastani 0000-0001-9789-1978 No Research Center of Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran Soraya Babaie 0000-0003-0259-2866 No Aging Research Institute, Physical Medicine, and Rehabilitation Research Centre, Tabriz University of Medical Sciences, Tabriz, Iran Amir Fattahi* 0000-0002-5712-5336 No Womenʼs Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Mahnaz Shahnazi 0000-0001-9676-8492 Yes Department of Midwifery, Faculty of Nursing and Midwifery, Tabriz University of Medical Sciences, Tabriz, Iran