en Cancer Biology Cancer Biology مقاله کامل Full Length/Original Article <div style="text-align: justify;"><span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:10.5pt"><span style="color:black">Background: </span></span></b><span style="font-size:10.5pt"><span style="color:black">This study investigated the role of the immune-checkpoint receptor (ICR), CD244, and its adapter molecules, in CD8⁺ T cells in acute leukemia.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:10.5pt"><span style="color:black">Methods</span></span></b><span style="font-size:10.5pt"><span style="color:black">: Blood samples were obtained from 21 acute lymphoblastic leukemia (ALL) and 6 acute myeloid leukemia (AML) patients and 20 control subjects. Relative gene expression of CD244, immune receptor tyrosine-based switch <em>motif-a</em>ssociated protein (SA), <span style="background:white">EWS/FLI1-activated transcript 2 (</span>EAT-2)</span></span><span dir="RTL" lang="FA" style="font-size:10.5pt"><span style="color:black">,</span></span><span style="font-size:10.5pt"><span style="color:black"> and LncRNA-GSTT1-AS1 were evaluated using </span></span><span style="font-size:10.5pt"><span style="color:black">quantitative reverse transcription polymerase chain reaction.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:10.5pt"><span style="color:black">Results: </span></span></b><span style="font-size:10.5pt"><span style="color:black">Expression of CD244, SAP, and EAT-2 were significantly lower in CD8⁺ T cells from ALL patients than those from control subjects. Interestingly, the expression of SAP was much lower than that of CD244, indicating a lower ratio of SAP to CD244. Also, SAP expression was significantly lower in AML patients compared to the control group. Expression of LncRNA-GSTT1-AS1 showed no significant difference in ALL and AML patients compared to control subjects.</span></span></span></span><br> <b><span style="font-size:10.5pt"><span style="line-height:115%"><span style="color:black">Conclusion: </span></span></span></b><span style="font-size:10.5pt"><span style="line-height:115%"><span style="color:black">The low SAP/CD244 expression ratio in CD8⁺ T cells in ALL suggests an inhibitory role for CD244 in ALL.</span></span></span> </span></div> CD244 Expression in Acute Leukemia 214 218 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-5163-1&slc_lang=en&sid=1 Maryam Mohammadi maryammohammadi513@gmail.com 0000-0002-2889-7177 No Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Hossein Asgarian-Omran asgarianhossein@yahoo.com 0000-0003-3816-8792 No Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Ahmad Najfi ahmad.najafi.2012@gmail.com 0000-0001-9821-336X No Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Reza Valadan valadan.reza@gmail.com 0000-0002-9074-6994 No Molecular and Cell-Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran Hossein Karami karami_oncologist@yahoo.com 0000-0001-9765-776X No Thalassemia Research Center (TRC), Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, Mazandaran Iran Mohammadd Naderisoraki dr.naderisorki@gmail.com 0000-0001-8638-4057 No Thalassemia Research Center (TRC), Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, Mazandaran Iran Ehsan Zaboli paya_1358@yahoo.com 0000-0003-4009-717X No Gastrointestinal Cancer Research Center, Mazandaran University of Medical Sciences, Sari, Iran Mohammad Eslami mo.eslami@mazums.ac.ir 0000-0001-5887-8869 No Gastrointestinal Cancer Research Center, Mazandaran University of Medical Sciences, Sari, Iran Mohsen Tehrani drmtehrani@gmail.com 0000-0002-9292-4520 Yes Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran