en Cancer Biology Cancer Biology مقاله کامل Full Length/Original Article <span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:10.5pt">Background:</span></b><span style="font-size:10.5pt"> Adenoid cystic carcinoma is a slow-growing malignancy that most often occurs in the salivary glands. Currently, no FDA-approved therapeutic target or diagnostic biomarker has been identified for this cancer. The aim of this study was to find new therapeutic and diagnostic targets using bioinformatics methods. </span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><b><span style="border:none windowtext 1.0pt; font-size:10.5pt; padding:0cm"><span style="background:white"><span style="color:black">Methods: </span></span></span></b><span style="font-size:10.5pt">We extracted the gene expression information from two GEO datasets (including GSE59701 and GSE88804). D</span>ifferent expression gene<span style="font-size:10.5pt">s between </span>adenoid cystic carcinoma<span style="font-size:10.5pt"> (ACC) and normal samples were extracted using R software. The biochemical pathways involved in ACC were obtained by using the Enrichr database. PPI network was drawn by STRING, and important genes were extracted by Cytoscape. Real-time PCR and immunohistochemistry were used for biomarker verification. </span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><b><span style="border:none windowtext 1.0pt; font-size:10.5pt; padding:0cm"><span style="background:white"><span style="color:black">Results:</span></span></span></b><span style="font-size:10.5pt"> After analyzing the PPI network, 20 hub genes were introduced to have potential as diagnostic and therapeutic targets. Among these genes, <i>PLCG1</i> was presented as new biomarker in ACC. <span style="color:black">Furthermore, by studying the function of the hub genes in the enriched biochemical pathways, we found that </span></span>insulin-like growth factor type 1 receptor<span style="font-size:10.5pt"><span style="color:black"> and PPARG pathways most likely play a critical role in tumorigenesis and drug resistance in ACC and have a high potential for selection as therapeutic targets in future studies. </span></span><b><span style="font-size:10.5pt"></span></b></span></span><br> <b><span style="font-size:10.5pt"><span style="line-height:115%">Conclusion:</span></span></b><span style="font-size:10.5pt"><span style="line-height:115%"><span style="color:black"> In this study, we achieved the recognition of the pathways involving in ACC pathogenesis and also found potential targets for treatment and diagnosis of ACC. Further experimental studies are required to confirm the results of this study.</span></span></span> </span> Adenoid cystic carcinoma, Adipogenesis, Biomarkers, IGF type 1 receptor 294 306 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-648-1&slc_lang=en&sid=1 Tayebeh Forooghi Pordanjani foroghd3@gmail.com 0000-0002-1452-7861 No Department of Biochemistry, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran Bahareh Dabirmanesh dabirmanesh2000@yahoo.com 0000-0002-3615-5683 No Department of Biochemistry, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran Peyman Choopanian choopanian.p1992@gmail.com 0000-0003-1428-9231 No Department of Applied Mathematics, Faculty of Mathematical Sciences, Tarbiat Modares University, Tehran, Iran Mehdi Mirzaie mirzaie@modares.ac.ir 0000-0003-4434-5118 No Department of Applied Mathematics, Faculty of Mathematical Sciences, Tarbiat Modares University, Tehran, Iran Saleh Mohebbi* mohebbi54@gmail.com 0000-0003-4050-7714 No ENT and Head & Neck Research Center, the Five Senses Health Institute, Rasoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran Khosro Khajeh khajeh_k@yahoo.com 0000-0001-6846-5048 Yes Department of Biochemistry, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran