Iranian Biomedical Journal
مجله بیومدیکال ایران
IBJ
Basic Sciences
http://ibj.pasteur.ac.ir
1
admin
1028-852X
2008-823X
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10.61882/ibj
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8888
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en
jalali
1401
12
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gregorian
2023
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27
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fulltext
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Comparison of Isoenzyme Pattern of Echinococcus granulosus sensu stricto (G1-G3) and E. canadensis (G6/G7) Protoscoleces
Molecular Microbiology
Molecular Microbiology
مقاله کامل
Full Length/Original Article
<div style="text-align: justify;"><span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:10.5pt">Background: </span></b><span style="font-size:10.5pt">Different genotypes of <i>Echinococcus granulosus sensu lato</i> (<i>s.l.</i>) infect humans and ungulate animals, causing cystic echinococcosis. Simultaneous isoenzyme, as well as molecular characterizations of this parasite, has not yet been investigated in Iran. The present study aimed to evaluate the isoenzyme pattern of the <i>E. granulosus </i>sensu stricto<i> </i>(<i>s.s.</i>)<i> </i>and <i>E. canadensis</i> genotypes in Iran.</span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:10.5pt">Methods</span></b><span style="font-size:10.5pt">: A total of 32 (8 humans and 24 <span style="color:black">animals) cystic echinococcosis cysts were isolated from Shiraz, Tehran, Ilam, and Birjand from May</span> 2018 to December 2020. The DNAs were extracted and their genotypes were determined by molecular methods. Enzymes were extracted from the cysts and subjected to polyacrylamide gel electrophoresis. The activities of </span><span lang="EN-GB" style="font-size:10.5pt"><span style="color:black">glucose-6-phosphate sehydrogenase</span></span><span style="font-size:10.5pt"> (G6PD), </span><span lang="EN-GB" style="font-size:10.5pt">malate dehydrogenase</span><span style="font-size:10.5pt"> (MDH), </span><span lang="EN-GB" style="font-size:10.5pt">malic enzyme</span> <span style="font-size:10.5pt">(ME), </span><span lang="EN-GB" style="font-size:10.5pt">nucleoside hydrolyse 1 (</span><span style="font-size:10.5pt">NH1), and </span><span lang="EN-GB" style="font-size:10.5pt">isocitrate dehydrogenase</span><span style="font-size:10.5pt"> (ICD) were examined in the cyst samples using isoenzyme method and compared it with the genotyping findings.</span> <span style="font-size:10.5pt"></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:10.5pt">Results:</span></b><span style="font-size:10.5pt"> DNA sequence analysis of the samples showed that the specimens contained 75% <i>E. granulosus s.s.</i> (G1) and 25% <i>E. canadensis</i> (G6) genotypes. The isoenzyme pattern of ICD in both genotypes produced a six-band pattern with different relative factors. The G6PD also produced two bands with different relative migrations in both genotypes. The MDH and NH1 systems revealed a two-band pattern, while only one band was generated in the ME enzyme in the <i>E. granulosus </i><i>s.s. g</i>enotype. In the <i>E. canadensis</i>, the MDH and NH1 enzymes showed one band, and the ME enzyme represented a two-band pattern.</span> <span style="font-size:10.5pt"></span></span></span><br>
<b><span style="font-size:10.5pt"><span style="line-height:107%">Conclusion: </span></span></b><span style="font-size:10.5pt"><span style="line-height:107%">Our findings suggest that <i>E. granulosus s.s.</i> and <i>E. canadensis</i> genotypes have entirely different isoenzyme patterns for NH1, G6PD, MDH, and ME</span></span><span style="font-size:10.5pt"><span style="line-height:107%">.</span></span></span></div>
Echinococcus granulosus, Genotype, Isoenzymes
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http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-3361-2&slc_lang=en&sid=1
Majid
Dousti
dousti6586@gmail.com