en Molecular Microbiology Molecular Microbiology مقاله کامل Full Length/Original Article <span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:normal"><span calibri=""><b><span style="font-size:12.0pt"><span new="" roman="" times="">Background: </span></span></b><i><span style="font-size:12.0pt"><span new="" roman="" times="">Cryptosporidium<b> </b>parvum</span></span></i><span style="font-size:12.0pt"><span new="" roman="" times=""> is an important coccidian parasite infecting many mammals, including human. This parasite can manifest as chronic severe diarrhea in immunocompromised individuals, especially those with AIDS. The present study reports the recombinant production of recombinant (r)P2 and rP23 antigens of <i>C. parvum</i> as antigens for detecting human cryptosporidiosis using indirect ELISA tests. </span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri=""><b><span style="font-size:12.0pt"><span new="" roman="" times="">Methods: </span></span></b><span style="font-size:12.0pt"><span new="" roman="" times="">The coding sequences of rP2 and rP23 proteins were codon-optimized, commercially synthesized and sub-cloned in the pET28a expression vector.</span></span> <span style="font-size:12.0pt"><span new="" roman="" times="">The expressed proteins were purified by Ni-NTA column chromatography and confirmed by Western blotting. The efficacy of rP2/rP23 proteins for serodiagnosis was evaluated by positive (n = 20) and negative (n = 20) human sera, confirmed by the Ziehl-Neelsen staining as the gold standard test. </span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri=""><b><span style="font-size:12.0pt"><span new="" roman="" times="">Results: </span></span></b><span style="font-size:12.0pt"><span style="background:white"><span new="" roman="" times=""><span style="color:black">In </span></span></span></span><span style="font-size:12.0pt"><span style="background:white"><span new="" roman="" times=""><span style="color:black">ELISA test, the sera from <i>C. parvum</i>-infected patients reacted strongly to rP2/rP23. The sensitivity and specificity related to the diagnostic potential of </span></span></span></span><span style="font-size:12.0pt"><span new="" roman="" times=""><span style="color:black">rP2/rP23<span style="background:white"> in the ELISA assay were </span>100</span></span></span><span style="font-size:12.0pt"><span new="" roman="" times="">%. </span></span></span></span></span><br> <b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" times="">Conclusion: </span></span></span></b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" times="">Our results showed that combination of rP23 and rP2 antigens in ELISA significantly increases the performance of <i>C. parvum </i>serodiagnosis in human cryptosporidiosis. </span></span></span></span> Cryptosporidiosis, Enzyme-Linked Immunosorbent Assay, Western blottingn 374 379 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-5076-1&slc_lang=en&sid=1 Gholamreza Barzegar Rezabarzegar51@gmail.com 0000-0002-3743-4318 No Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran Ehsan Ahmadpour Ehsanahmadpour@gmail.com 0000-0003-1202-6147 Yes Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Bahador Shahriari sarkarib@sums.ac.ir 0000-0003-2045-9057 No Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran Rahmat Solgi Rahmatsolgi@yahoo.com 0000-0002-2695-7658 No Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran Mohammad Hossein Motazedian Motazedian@SUMS.ac.ir 0000-0003-0117-9666 No Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran