en Medical Biotechnology Medical Biotechnology مقاله کامل Full Length/Original Article <span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:normal"><span calibri=""><b><span style="font-size:12.0pt"><span new="" roman="" times=""><span style="color:black">Introduction:</span></span></span></b><span style="font-size:12.0pt"><span new="" roman="" times=""><span style="color:black"> Brain ischemia often leads to the chloride gradient alternations, which affects volume regulation and neuronal survival. Increase in NKCC1 expression and reduction in KCC2 level under ischemic condition results in inflammation and </span></span></span><span style="font-size:12.0pt"><span new="" roman="" times=""><span style="color:black">neuronal death. In this study, we investigated the effect of mimic miRNA and </span></span></span><span style="font-size:12.0pt"><span new="" roman="" times="">coenzyme Q10 (<span style="color:black">CoQ10) on the expression of </span>cation-chloride cotransporters<span style="color:black"> (CCCs) (NKCC1 and KCC2) after cerebral ischemia. </span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri=""><b><span style="font-size:12.0pt"><span new="" roman="" times=""><span style="color:black">Methods:</span></span></span></b> <span style="font-size:12.0pt"><span new="" roman="" times="">In this study, cerebral ischemia was modeled using the </span></span><span style="font-size:12.0pt"><span new="" roman="" times="">middle cerebral artery occlusion</span></span><span style="font-size:12.0pt"><span new="" roman="" times=""> method. Rats were randomly divided into six groups: sham, model, </span></span><span style="font-size:12.0pt"><span new="" roman="" times="">negative control</span></span><span style="font-size:12.0pt"><span new="" roman="" times="">, vehicle, and the first and second treatments. In the Sham group, ischemia was not induced, and no treatment was performed. In the Model group, ischemia induction was performed, and other groups, in addition to ischemia induction, received Scramble miRNA, </span></span><span style="font-size:12.0pt"><span new="" roman="" times=""><span style="color:black">Ethanol</span></span></span><span style="font-size:12.0pt"><span new="" roman="" times="">, mimic miRNA-149-5p and CoQ10, respectively. </span></span><span style="font-size:12.0pt"><span new="" roman="" times=""><span style="color:black">Each group was divided into three subgroups to assess the </span></span></span><span style="font-size:12.0pt"><span new="" roman="" times=""><span style="color:black">volume of the tissue damage and </span></span></span><span style="font-size:12.0pt"><span new="" roman="" times=""><span style="color:black">neurological deficits scores</span></span></span><span style="font-size:12.0pt"><span new="" roman="" times=""><span style="color:black"> (NDS) in subgroup 1, brain water content in subgroup 2, level of miRNA-149-5p and CCC expressions in subgroup 3. </span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri=""><b><span style="font-size:12.0pt"><span new="" roman="" times=""><span style="color:black">Results:</span></span></span></b><span style="font-size:12.0pt"><span new="" roman="" times=""><span style="color:black"> Our data suggested that the use of mimic miRNA and Q10 increased the level of miRNA-149 and KCC2 expression and decreased NDS, NKCC1 expression, brain water content, and infract volume. </span></span></span></span></span></span><br> <b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" times=""><span style="color:black">Conclusion:</span></span></span></span></b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" times=""><span style="color:black"> Findings of this study suggest that the mimic miRNA and Q10 may have neuroprotective effects through reducing infract volume and brain water content and modulating the expression of CCCs </span></span></span></span><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" times=""><span style="color:black">after brain ischemia.</span></span></span></span></span> Brain edama, Brain ischemia, Coenzyme Q10, MicroRNAs 357 365 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-4986-1&slc_lang=en&sid=1 Hossein Mostafavi mostafavih@zums.ac.ir 0000-0002-4784-4636 No Department of Physiology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran. Narges Amoli nargesamm@yahoo.com 0000-0003-4096-6064 No Department of Physiology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran. Elham Ghasemloo ghasemloo_e@yahoo.com 0000-0002-3278-4570 No Department of Physiology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran. Meysam Forouzandeh foroozandeh.meysam67@gmail.com 0000-0003-3069-3189 No Faculty of Life Sciences, Shahid-Beheshti University, Tehran, Iran. Masoumeh Hosseini mahozanjani@yahoo.com 0000-0001-5018-2437 No Laboratory Expert Depertment of Physiology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran. Mehdi Eskandari mehdiesk@zums.ac.ir 0000-0002-8569-6486 Yes Department of Physiology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.