en Cancer Biology Cancer Biology مقاله کامل Full Length/Original Article <span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:normal"><span calibri=""><b><span style="font-size:10.5pt">Introduction: </span></b><span style="font-size:10.5pt">Chemotherapy, biotherapy, and radiotherapy play a limited but important role in treating breast cancer. For more efficient treatment, combination therapy could be an appropriate option. In this study, radiotherapy using neutron radiation emitted from a ²⁴¹Am-Be neutron source, as well as biotherapy using curcumin (80 &mu;M) was combined to investigate the efficiency of treatment towards MCF-7 breast cancer in a </span><span style="font-size:10.5pt">three dimensional</span><span style="font-size:10.5pt"> (3D) culture medium. </span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri=""><b><span style="font-size:10.5pt">Methods:</span></b><span style="font-size:10.5pt"> MTT, </span><span style="font-size:10.5pt">neutral red</span><span style="font-size:10.5pt"> uptake assay, </span><span style="font-size:10.5pt">nitric oxide</span><span style="font-size:10.5pt">, </span><span style="font-size:10.5pt">glutathione</span><span style="font-size:10.5pt"> assay, catalase, cytochrome c, comet assay, and caspase-3 were used to determine the effect of neutron radiation and also neutron and curcumin combination on the viability of cancer cells. </span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri=""><b><span style="font-size:10.5pt">Results: </span></b><span style="font-size:10.5pt">The results of cytotoxicity test showed that neutron irradiation with or without curcumin at 5, 10, 15, and 20 h reduced the survival of tumor cells. Moreover, the rate of apoptosis due to&nbsp;the&nbsp;neutron effect at different irradiation times enhanced with the increasing time. </span></span></span></span><br> <b><span style="font-size:10.5pt"><span style="line-height:115%"><span calibri="">Conclusion: </span></span></span></b><span style="font-size:10.5pt"><span style="line-height:115%"><span calibri="">Due to the significant anticancer effect of curcumin in 3D culture, using this molecule before or after neutron therapy is recommended.</span></span></span></span> Breast neoplasms, Curcumin, MCF-7 cells 475 484 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-4908-1&slc_lang=en&sid=1 Sajedeh Zargan zzargan.2013@gmail.com 0000-0001-5501-5769 No Department of Medical Radiation Engineering, Central Tehran Branch, Islamic Azad University, Tehran, Iran Mahdi Salehi Barough msbarough@iauctb.ac.ir 0000-0002-5680-0002 Yes Department of Medical Radiation Engineering, Central Tehran Branch, Islamic Azad University, Tehran, Iran Jamil Zargan jzargan@yahoo.com 0000-0002-1655-4265 No Department of Biology, Faculty of Basic Sciences, Imam Hossein Comprehensive University, Tehran, Iran Mohsen Shayesteh mshayesteh@ihu.ac.ir 0000-0002-5771-9708 No Department of Medical Radiation Engineering, Central Tehran Branch, Islamic Azad University, Tehran, Iran Ashkan Haji Noor Mohammadi ashkan.genetics@gmail.com 0000-0002-2420-4716 No Department of Biology, Faculty of Basic Sciences, Imam Hossein Comprehensive University, Tehran, Iran Mohsen Mousavi smohsenmousavi71@gmail.com 0000-0002-5984-0739 No Department of Biology, Faculty of Basic Sciences, Imam Hossein Comprehensive University, Tehran, Iran Hani Keshavarz Alikhani alikhani865@gmail.com 0000-0001-7005-5072 No Faculty of Science, Department of Biology, Razi University, Kermanshah, Iran