Iranian Biomedical Journal
مجله بیومدیکال ایران
IBJ
Basic Sciences
http://ibj.pasteur.ac.ir
1
admin
1028-852X
2008-823X
-
10.61186/ibj
-
8888
-
en
jalali
1401
8
1
gregorian
2022
11
1
26
6
online
1
fulltext
en
Osteogenic Differentiation Effect of BMP-9 with Phenamil and Simvastatin on Intact Human Amniotic Epithelial Stem Cells
Tissue Engineering and Cell Biology
Tissue Engineering and Cell Biology
مقاله کامل
Full Length/Original Article
<span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:normal"><span calibri=""><b><span style="font-size:10.5pt">Background: </span></b><span style="font-size:10.5pt">Bone tissue engineering has shown to be a promising strategy for repairing bone defects without causing harmful side effects to the patient. Three main building blocks of tissue engineering, including seeding cells, scaffold, and signaling molecules, are required for adequate bone regeneration. The </span><span style="font-size:10.5pt">human amniotic membrane</span><span style="font-size:10.5pt"> (hAM) is the innermost of the placental membranes. In addition to providing a source of stem cells and growth factors, hAM has several features that make it an appropriate scaffold containing stem cells for use in tissue engineering purposes. The present investigation aimed to assess the effect of bone morphogenetic protein-9 (BMP-9) combined with phenamil and simvastatin on osteogenic induction of hAM with its </span><span style="font-size:10.5pt">human amniotic membrane epithelial cells</span><span style="font-size:10.5pt"> (hAECs). </span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span calibri=""><b><span style="font-size:10.5pt">Methods:</span></b><span style="font-size:10.5pt"> Using six different </span><span style="font-size:10.5pt">osteogenic medium</span><span style="font-size:10.5pt"> (OMs), we cultured hAM for 14 days. The basic OMs were chosen as the first group and other media were made by adding BMP-9, phenamil, simvastatin, BMP-9 alongside phenamil, and BMP-9 alongside simvastatin to the basic OMs. Finally, viability assay, tissue mineralization, calcium and phosphate content determination, and measurement of </span><span style="font-size:10.5pt">lactic acid dehydrogenase</span><span style="font-size:10.5pt"> (LDH), and </span><span style="font-size:10.5pt">alkaline phosphatase</span><span style="font-size:10.5pt"> (ALP) activity were performed. </span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span calibri=""><b><span style="font-size:10.5pt">Results:</span></b><span style="font-size:10.5pt"> Among all study groups, groups containing simvastatin showed a significantly lower level of viability. Although all media could induce osteogenic features, the hAECs cultured in media containing BMP-9 and phenamil demonstrated a wider area of mineralization and a significantly higher level of calcium and</span> <span style="font-size:10.5pt">phosphate content</span><span style="font-size:10.5pt">, LDH, and ALP activity. </span></span></span></span><br>
<b><span style="font-size:10.5pt"><span style="line-height:115%"><span calibri="">Conclusion:</span></span></span></b><span style="font-size:10.5pt"><span style="line-height:115%"><span calibri=""> Our findings indicated that the use of phenamil together with BMP-9 could synergistically show <i>in situ</i> osteogenic induction in hAECs, which could be a new insight into translational medicine.</span></span></span></span>
Amniotic Membrane, Phenamil, Simvastatin, Tissue engineering
463
474
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-4958-1&slc_lang=en&sid=1
Armin
Ahmadi
arminahmadi@sbmu.ac.ir
100319475328460059834
100319475328460059834
No
Department of Pharmacology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Seyed Shayan
Ebadi
ss.ebadi@sbmu.ac.ir
100319475328460059835
100319475328460059835
No
Department of Pharmacology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Tahereh
Tayebi
tayebi_t@sbmu.ac.ir
100319475328460059836
100319475328460059836
No
Department of Pharmacology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Seyed Alireza
Ebadi
sa.ebadi@sbmu.ac.ir
100319475328460059837
100319475328460059837
No
Department of Internal Medicine, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Mohammad Mahdi
Sarzaeem
100319475328460059838
100319475328460059838
No
Department of Orthopedic Surgery and Traumatology, Imam Hossein Medical Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Hassan
Niknejad
niknejad@sbmu.ac.ir
100319475328460059839
100319475328460059839
Yes
Department of Pharmacology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran