en اثر سیتوتوکسیک آلبومین گلیکه- یون آلومینیوم بر روی سوسپانسیون سلولی کبد رت مقاله کامل Full Length/Original Article <p><font face="Arial" size="3"><font face="Arial" size="3"><font face="times new roman,times,serif"><font size="2"><span style="FONT-SIZE: 11pt mso-bidi-language: FA"><font face="times new roman,times,serif" size="2"><strong>Background</strong></font></span><span><font face="times new roman,times,serif" size="2"><strong>:</strong> Combination of glycation and oxidation is associated with diabetes mellitus. The aim of this study was to clarify the effect of glycated proteins in presence of transition metal ions on production of reactive oxygen species (ROS) in rat hepatocyte suspension. <span style="mso-spacerun: yes">&nbsp;</span><strong>Methods:</strong> Glycated albumin was prepared by incubation of bovine serum albumin with</font> <font face="times new roman,times,serif" size="2">100 mM glucose in 0.3 M phosphate buffer at 37</font><font face="times new roman,times,serif" size="2">&deg;</font></span><span style="FONT-SIZE: 11pt mso-bidi-language: FA"><font face="times new roman,times,serif" size="2">C for 2, 4 , and 6 weeks. The prepared rat liver cell suspension was treated with glycated albumin in presence of either Fe⁺⁺⁺ or Cu⁺⁺ ions. Produced malone-dialdehyde was measured as an indicator of ROS and of cell injury.<span style="mso-spacerun: yes">&nbsp; </span><strong>Results:</strong> The results showed Fe⁺⁺⁺ and Cu⁺⁺ ions increase the ROS production in presence of glycated albumin (<i>p</i><0.01). All prepared glycated albumin showed cytotoxicity in rat hepatocytes suspension in the presence of cupric and ferric ions, and this injury was dependent to metal ion concentration. Higher degree of glycation showed higher effect on ROS production (<i>p</i><0.01). Comparing the effect of Fe⁺⁺⁺ and Cu⁺⁺, cupric ion had higher cytotoxic effect (<i>p</i><0.01).<span style="mso-spacerun: yes">&nbsp; </span><strong>Conclusion:</strong> These results indicated that hepatocytes</font> <font face="times new roman,times,serif" size="2">may be damaged by ROS which are produced by the interaction of the glycated albumin and transition metal ion.</font></span></font></font></font></font></p> Albumin, Glycation, Reactive oxygen species (ROS), Transition metal ion 139 143 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-132&slc_lang=en&sid=1 Mohammad Taghi Goodarzi محمدتقی گودرزی mtgoodarzi@yahoo.com Yes Mohammad reza Safari محمدرضا صفری No Fatemeh Zal فاطمه زال No