Iranian Biomedical Journal

en A Signature of Three microRNAs Is a Potential Diagnostic Biomarker for Glioblastoma Cancer Biology Cancer Biology مقاله کامل Full Length/Original Article Background: Glioblastoma is the most common primary malignant neoplasm of the central nervous system. Despite progress in diagnosis and treatment, glioblastoma still has a poor prognosis. This study aimed to examine whether a signature of three candidate miRNAs (i.e. hsa-let-7c-5p, hsa-miR-206-5p, and hsa-miR-1909-5p) can be used as a diagnostic biomarker for distinguishing glioblastoma from healthy brain tissues. Methods: In this study, 50 formalin‐fixed paraffin‐embedded (FFPE) glioblastoma tissue samples and 50 healthy tissue samples adjacent to tumor were included. The expression of each candidate miRNA (i.e. hsa-let-7c-5p, hsa-miR-206-5p, and hsa-miR-1909-5p) was measured using quantitative reverse transcription PCR. To show the roles of each miRNA and their biological effects on glioblastoma development and clinicopathological characteristics, in silico tools were used. ROC curves were performed to assess the diagnostic accuracy of each miRNA. Results: Based on the results, hsa-let-7c-5p and hsa-miR-206-5p were downregulated, while hsa-miR-1909-5p was upregulated in glioblastoma tumors compared to healthy samples. No association was detected between the expression of each candidate miRNA and sex. Except for hsa-let-7c-5p, other miRNAs did not correlate with age status. ROC curve analysis indicated that the signature of candidate miRNAs is a potential biomarker distinguishing between glioblastoma and healthy samples. Only hsa-miR-206-5p suggested the association with poor prognosis in glioblastoma patients. Conclusion: Our findings revealed that the signature of three miRNAs is capable of distinguishing glioblastoma tumor and healthy tissues. These results are beneficial for the clinical management of glioblastoma patients. Inflammation, Gastric cancer, Gene expression, Pattern recognition receptors, RNA-seq 301 312 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-4855-1&slc_lang=en&sid=1 Ali Hosein Yazdi hoseinyazdiali@gmail.com 0000-0002-5257-9443 No Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran Vajiheh Zarrinpour zarrinpour_v@yahoo.com 0000-0002-5257-9443 Yes Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran Elham Moslemi emoslemi@iauet.ac.ir 0000-0002-1473-4541 No Cellular and Molecular Biology Department, Islamic Azad University, Tehran East Branch, Tehran, Iran Mohammad Mahdi Forghanifard Forghanifard@gmail.com 0000-0002-7691-6897 No Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran