Iranian Biomedical Journal
مجله بیومدیکال ایران
IBJ
Basic Sciences
http://ibj.pasteur.ac.ir
1
admin
1028-852X
2008-823X
-
10.61186/ibj
-
8888
-
en
jalali
1401
4
1
gregorian
2022
7
1
26
4
online
1
fulltext
en
A Signature of Three microRNAs Is a Potential Diagnostic Biomarker for Glioblastoma
Cancer Biology
Cancer Biology
مقاله کامل
Full Length/Original Article
<span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:normal"><span calibri=""><b><span style="font-size:10.5pt">Background:</span></b> <span style="font-size:10.5pt">Glioblastoma is the most common primary malignant neoplasm of the central nervous system. Despite progress in diagnosis and treatment, glioblastoma still has a poor prognosis. This study aimed to examine whether a signature of three candidate miRNAs (i.e. hsa-let-7c-5p, hsa-miR-206-5p, and hsa-miR-1909-5p) can be used as a diagnostic biomarker for distinguishing glioblastoma from healthy brain tissues. </span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span calibri=""><b><span style="font-size:10.5pt">Methods:</span></b> <span style="font-size:10.5pt">In this study, 50 formalin‐fixed paraffin‐embedded (FFPE) glioblastoma tissue samples and 50 healthy tissue samples adjacent to tumor were included. The expression of each candidate miRNA (i.e. hsa-let-7c-5p, hsa-miR-206-5p, and hsa-miR-1909-5p) was measured using quantitative reverse transcription PCR. To show the roles of each miRNA and their biological effects on glioblastoma development and clinicopathological characteristics, <i>in silico </i>tools were used. ROC curves were performed to assess the diagnostic accuracy of each miRNA. </span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span calibri=""><b><span style="font-size:10.5pt">Results:</span></b> <span style="font-size:10.5pt">Based on the results, hsa-let-7c-5p and hsa-miR-206-5p were downregulated, while hsa-miR-1909-5p was upregulated in glioblastoma tumors compared to healthy samples. No association was detected between the expression of each candidate miRNA and sex. Except for hsa-let-7c-5p, other miRNAs did not correlate with age status. ROC curve analysis indicated that the signature of candidate miRNAs is a potential biomarker distinguishing between glioblastoma and healthy samples. Only hsa-miR-206-5p suggested the association with poor prognosis in glioblastoma patients. </span></span></span></span><br>
<b><span style="font-size:10.5pt"><span style="line-height:115%"><span calibri="">Conclusion:</span></span></span></b> <span style="font-size:10.5pt"><span style="line-height:115%"><span calibri="">Our findings revealed that </span></span></span><span style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" times="">the signature of three miRNAs is capable of distinguishing glioblastoma tumor and healthy tissues</span></span></span><span style="font-size:10.5pt"><span style="line-height:115%"><span calibri="">. </span></span></span><span style="font-size:10.5pt"><span style="line-height:115%"><span calibri="">These results are beneficial for the clinical management of glioblastoma patients. </span></span></span></span>
Inflammation, Gastric cancer, Gene expression, Pattern recognition receptors, RNA-seq
301
312
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-4855-1&slc_lang=en&sid=1
Ali
Hosein Yazdi
hoseinyazdiali@gmail.com
100319475328460053931
100319475328460053931
No
Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
Vajiheh
Zarrinpour
zarrinpour_v@yahoo.com
100319475328460053932
100319475328460053932
Yes
Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
Elham
Moslemi
emoslemi@iauet.ac.ir
100319475328460053933
100319475328460053933
No
Cellular and Molecular Biology Department, Islamic Azad University, Tehran East Branch, Tehran, Iran
Mohammad Mahdi
Forghanifard
Forghanifard@gmail.com
100319475328460053934
100319475328460053934
No
Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran