en تشکیل غدد با روغن معدنی در کشت سلول‌های استرومای مغز استخوان توسط فیبرویین مقاله کامل Full Length/Original Article <p align="justify"><font face="Arial" size="3"><font face="Arial" size="3"><span style="FONT-SIZE: 11pt"><span><font size="2"><font face="times new roman,times,serif"><span style="FONT-FAMILY: "><strong><font face="times new roman,times,serif">Background:</font></strong></span><span style="FONT-FAMILY: "><font face="times new roman,times,serif"> Silk fibroin is a suitable protein for osteogenesis by inducing markers of bone formation in the cultures of osteoblasts, so we examined the ability of this protein to induce mineralized nodules in the rat bone marrow stromal cell cultures. <b>Methods:</b> Bone marrow stromal cells obtained from 4 to 6 weeks old Spruge-Dawely male rats were grown in primary culture for seven days and then subcultured fo</font>r <font face="times new roman,times,serif">21 days. The secondary cultures were done on either silk fibroin-coated polystyrene plates or free-silk fibroin ones. </font></span><font face="times new roman,times,serif"><span style="FONT-FAMILY: ">After 21 days of grow up, the cultures were examined for </span><span style="FONT-FAMILY: ">nodule formation by scanning electron microscopy, for mineralization </span><span style="FONT-FAMILY: ">by </span><span style="FONT-FAMILY: ">alizarin red </span><span style="FONT-FAMILY: ">S staining and </span></font><span style="FONT-FAMILY: "><font face="times new roman,times,serif">for expression of gene markers of osteoblast maturation by</font> </span></font><font face="times new roman,times,serif"><span style="FONT-FAMILY: "><font face="times new roman,times,serif">reverse</font> <font face="times new roman,times,serif">transcription<b><i>&nbsp;</i></b></font></span></font><font face="times new roman,times,serif"><span style="FONT-FAMILY: ">PCR (RT-PCR</span></font><span style="FONT-FAMILY: "><font face="times new roman,times,serif">).</font><font face="Times New Roman"><font face="times new roman,times,serif"><span style="mso-spacerun: yes">&nbsp; </span><b>Results: </b>The stromal cells were observed to form three-dimensional nodules when cultured on the silk fibroin and compared to the stromal cells cultured on the free-silk fibroin polystyrene plates, where no nodules were observed in the time-frame studied. These nodules were also found to be mineralized and expressed the gene markers of osteoblast.<span style="COLOR: black"> <b><font face="times new roman,times,serif">Conclusion:</font></b> </span>Silk fibroin could serve as suitable inducing factor by <span style="COLOR: black">stimulating stromal cell differentiation to form </span>mineralized no</font>dules.</font></span></font></span></span></font></font></p> Bone marrow stromal cells, Mineralization, Fibroin, Culture 133 138 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-131&slc_lang=en&sid=1 Mehdi Nikbakht Dastjerdi مهدی نیکبخت دستجردی Yes