Iranian Biomedical Journal
مجله بیومدیکال ایران
IBJ
Basic Sciences
http://ibj.pasteur.ac.ir
1
admin
1028-852X
2008-823X
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10.61186/ibj
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8888
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en
jalali
1385
4
1
gregorian
2006
7
1
10
3
online
1
fulltext
en
بررسی تکثیر سلولهای سرطانی سینه رده MCF-7 کشت تورم با سلولهای آدیپوسایت انسانی در محیط کشت ماتریکس ژلهای کلاژن سه بعدی با تاکید بر نوراپی نفرین به عنوان یک فاکتور لیپولیتیک
Evaluation of Human Breast Adenocarcinoma (MCF-7) Cells Proliferation in Co-Culture with Human Adipocytes in Three Dimensional Collagen Gel Matrix: Norepinephrine as a Lipolytic Factor
مقاله کامل
Full Length/Original Article
<font face="times new roman,times,serif" size="2"><strong>Background:</strong> Norepinephrine plays a trophic role in the control of cell replication and differentiation in target cells that express adrenergic receptors. <strong>Methods:</strong> In this study, we have tested the influence of infraphysiological, physiological and supraphysiological concentrations (0.0001 nM, 1 nM, 10000 nM) of human norepinephrine on the proliferation of breast cancer cells (human breast adenocarcinoma [MCF-7]) in co-culture with human adipocytes in three-dimensional collagen gel matrix culture. Cell proliferation and lipolysis rate were measured by 3-(4, 5-dimethylthiazolyl)-2, 5-diphenyl-tetrazolium bromide (MTT) and Oil red O colorimetric assay in second, 7th days of culture experiments. <strong>Results:</strong> Our results showed a direct correlation between lipolysis rate of adipocytes and proliferation rate of MCF-7 cells. Both physiological and supraphysiological concentrations of human norepinephrine significantly (P<0.05) increased the proliferation of MCF-7 cells synchronously with progress of adipocyte lipolysis. The proliferations of MCF-7 cells were significantly decreased after conversion of adipocytes to fibroblast-like cells by supraphysiological concentration of norepinephrine. There was no statistical difference in lipolysis of adipocytes and proliferation of MCF-7 cells in response to infraphysiological concentration of norepinephrine. <strong>Conclusion:</strong> These findings indicated that norepinephrine stimulated the proliferation of MCF-7 cells in coculture with human adipocytes as a lipolytic factor and that norepinephrine effect was suppressed by conversion of adipocytes to fibroblast-like cells, suggesting adipocytes as another target for prevention and therapy of breast cancer. </font>
Human breast adenocarcinoma (MCF-7) cell, Human adipocyte, Norepinephrine, Proliferation, Lipolysis
125
131
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-130&slc_lang=en&sid=1
Mohammad
Barbarestani
محمد
بربرستانی
barbarestani@tums.ac.ir
100319475328460057260
100319475328460057260
Yes
Iraj
Ragerdi Kashani
ایرج
راگردی کاشانی
100319475328460057261
100319475328460057261
No
Farideh
Etesam
فریده
اعتصام
100319475328460057262
100319475328460057262
No
Mohammad Ali
Shokrgozar
محمدعلی
شکرگزار
100319475328460057263
100319475328460057263
No
Mir Abbas
Abdolvahabi
میرعباس
عبدالوهابی
100319475328460057264
100319475328460057264
No
Peiman
Haddad
پیمان
حداد
100319475328460057265
100319475328460057265
No
Mohammad Hossein
Noori Mokohi
محمدحسین
نوری موکوهی
100319475328460057266
100319475328460057266
No
Mostafa
Hosseni
مصطفی
حسینی
100319475328460057267
100319475328460057267
No