Iranian Biomedical Journal

en Regulation of Lysozyme Activity by Human Hormones Enzymology and Protein Chemistry Enzymology and Protein Chemistry مقاله کامل Full Length/Original Article <div style="text-align: justify;">Background: Lysozyme is a part of human and animal noncellular immunity. The regulation of its activity by hormones is poorly studied. The aim of this study was to test the in vitro activity of lysozyme in the presence of catecholamines, natriuretic hormones, and estradiol (E2). Methods: Hormones were incubated with lysozyme, and the activity of lysozome was further determined using a test culture of Micrococcus luteus in the early exponential growth stage. The activity of lysozyme was assessed based on the rate of change in the OD of the test culture. Molecular docking was performed using SwissDock server (http://www.swissdock.ch/docking), and molecular structures were further analyzed and visualized in the UCSF Chimera 1.15rc software. Results: According to the results, epinephrine and norepinephrine increased lysozyme activity up to 180% compared to the hormone-free enzyme. Changing the pH of the medium from 6.3 to 5.5, increased the lysozyme activity in the presence of E2 up to 150-200 %. The results also showed that exposure to hormones could modify lysozyme activity, and this effect depends on the temperature and pH value. The molecular docking revealed a decrease in the activation energy of the active site of enzyme during the interaction of catecholamines with the amino acid residues, asp52 and glu35 of the active site. Conclusion: Our findings demonstrate an additional mechanism for the involvement of lysozyme in humoral regulation of nonspecific immunity with respect to human pathogenic microflora and bacterial skin commensals by direct modulation of its activity using human hormones.</div> Catecholamines, Estradiol, Muramidase, Natriuretic peptides 58 65 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-4786-1&slc_lang=en&sid=1 Timofei A. Pankratov tpankratov@gmail.com 0000-0002-6636-6449 Yes S.N.Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow 119071, Russia Andrey V. Gannesen andrei.gannesen@gmail.com 0000-0002-8357-7205 No S.N.Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow 119071, Russia Yuri A. Nikolaev nikolaevya@mail.ru 0000-0002-2264-6764 No S.N.Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow 119071, Russia