Iranian Biomedical Journal
مجله بیومدیکال ایران
IBJ
Basic Sciences
http://ibj.pasteur.ac.ir
1
admin
1028-852X
2008-823X
-
10.61186/ibj
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8888
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en
jalali
1401
4
1
gregorian
2022
7
1
26
4
online
1
fulltext
en
MiR-106b-5p Regulates the Reprogramming of Spermatogonial Stem Cells into iPSC (Induced Pluripotent Stem Cell)-Like Cells
Molecular Genetics & Genomics
Molecular Genetics & Genomics
مقاله کامل
Full Length/Original Article
<span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:normal"><span calibri=""><b><span style="font-size:10.5pt">Background:</span></b> <span style="font-size:10.5pt">Recent years have brought notable progress in raising the efficiency of the reprogramming technique so that approaches have evolved from known transgenic factors to only a few miRNAs. Nevertheless, there is a poor understanding of both the key factors and biological networks underlying this reprogramming. The present study aimed to investigate the potential of miR-106b-5p in regulating spermatogonial stem cells (SSCs) to induced pluripotent stem cell (iPSC)-like cells. </span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span calibri=""><b><span style="font-size:10.5pt">Methods:</span></b> <span style="font-size:10.5pt">We used SSCs because pluripotency is inducible in SSCs under defined culture conditions, and they have a few issues compared to other adult stem cells. As both signaling and post-transcriptional gene controls are critical for pluripotency regulation, we traced the expression of <i>Oct-4</i>, <i>Sox-2</i>, <i>Klf-4</i>, <i>c-Myc</i>, and <i>Nanog</i> (OSKMN). Besides, we considered miR-106b-5p targets using bioinformatic methods. </span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span calibri=""><b><span style="font-size:10.5pt">Results:</span></b> <span style="font-size:10.5pt">Our results showed that transfected SSCs with miR-106b-5p increased the expression of the OSKMN factors, which was significantly more than negative control groups. Moreover, using the functional miRNA enrichment analysis, online tools, and databases, we predicted that miR-106b-5p targeted a signaling pathway gene named <i>MAPK1/ERK2</i>, related to regulating stem cell pluripotency. </span></span></span></span><br>
<b><span style="font-size:10.5pt"><span style="line-height:115%"><span calibri="">Conclusion:</span></span></span></b> <span style="font-size:10.5pt"><span style="line-height:115%"><span calibri="">Together, our data suggest that miR-106b-5p regulates the reprogramming of SSCs into iPSC-like cells. Furthermore, noteworthy progress in the <i>in vitro</i> development of SSCs indicates promise reservoirs and opportunities for future clinical trials. </span></span></span></span>
Computational biology, MicroRNAs, Pluripotent stem cells, Signal transduction
291
300
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-4759-1&slc_lang=en&sid=1
Amir Hossein
Hasani Fard
hfdamir@yahoo.com
100319475328460054290
100319475328460054290
No
Men’s Health and Reproductive Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Mahmoud
Valizadeh
dmv313140@gmail.com
100319475328460054291
100319475328460054291
No
Men’s Health and Reproductive Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Zohreh
Mazaheri
mazaheri2020@yahoo.com
100319475328460054292
100319475328460054292
No
Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Jalil
Hosseini
jhosseinee@gmail.com
100319475328460054293
100319475328460054293
Yes
Men’s Health and Reproductive Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran