Iranian Biomedical Journal
مجله بیومدیکال ایران
IBJ
Basic Sciences
http://ibj.pasteur.ac.ir
1
admin
1028-852X
2008-823X
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10.61186/ibj
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8888
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en
jalali
1400
10
1
gregorian
2022
1
1
26
1
online
1
fulltext
en
TLR2 and TLR4 Signaling Pathways and Gastric Cancer: Insights from Transcriptomics and Sample Validation
Cancer Biology
Cancer Biology
مقاله کامل
Full Length/Original Article
<strong>Background:</strong> Pattern recognition receptors, especially toll-like receptors (TLRs), as the first line of defense for pathogen detection, were found to be associated with <em>H.­ pylori </em>infection and gastric cancer (GC). However, the expression levels of TLRs, i.e. TLR2 and TLR4, as the main receptors sensed by <em>H.­ pylori</em>, still remain largely ambiguous. We aimed to investigate the patterns of key transcripts of TLR2 and TLR4 in 100 GC transcriptome data. Additionally, we evaluated TLR2 and TLR4 gene expressions in gastric biopsies of Iranian GC patients, in order to validate RNA-seq outputs. <strong>Methods:</strong> For this study, 100 runs of GC samples and controls were processed and analyzed using map read to reference. Differential gene expression method was used to distinguish between GC and normal samples in the expression of TLRs and other innate immune molecules. Also, using qRT-PCR assay, transcripts of TLRs molecules for 15 GC and 15 control samples were analyzed based on the analysis of variance and least significant differences. <strong>Results:</strong> The results clearly showed that all signaling pathways molecules of TLR4, especially TLR4 (<em>p</em> = 0.019), NF-κB (<em>p</em> ­= 0.047), IL-1β (<em>p</em> = 0.0096), and TNF-α (<em>p</em> = 0.048), were upregulated in a cancerous condition in different parts and at various stages of GC. <strong>Conclusion:</strong> Our findings suggested that molecules involved in inflammation, including TLR4 and its related pro-inflammatory cytokines, may be responsible for the development and progression of GC. Accordingly, the control of<em> H. pylori</em> infection reduces inflammation in the gastric system and can play an important role in preventing gastrointestinal disorders.
Inflammation, Gastric cancer, Gene expression, Pattern recognition receptors, RNA-seq
36
43
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-4663-1&slc_lang=en&sid=1
Saeed
Zargari
saeedzargari206@yahoo.com
100319475328460053989
100319475328460053989
No
Department of Biology, Hamedan Branch, Islamic Azad University, Hamedan, Iran
Abbas
Bahari
bahari@znu.ac.ir
100319475328460053990
100319475328460053990
Yes
Research Institute of Modern Biological Techniques, University of Zanjan, Zanjan, Iran
MohammadTaghi
Goodarzi
mtgoodarzi@yahoo.com
100319475328460053991
100319475328460053991
No
Department of Biochemistry, Shahrood Branch, Islamic Azad University, Shahrood, Iran
Minoo
Mahmoodi
minoomahmoodi@yahoo.com
100319475328460053992
100319475328460053992
No
Department of Biology, Hamedan Branch, Islamic Azad University, Hamedan, Iran
Reza
Valadan
reza.valadan@gmail.com
100319475328460053993
100319475328460053993
No
Department of Immunology, Molecular and Cell Biology Research Center, School of Medicine, Mazandaran University of Medical science, Mazandaran, Iran