Iranian Biomedical Journal

en HOTAIR Induces the Downregulation of miR-200 Family Members in Gastric Cancer Cell Lines Molecular Genetics & Genomics Molecular Genetics & Genomics مقاله کامل Full Length/Original Article Background: Gastric cancer (GC) is the fourth most common human malignancy and the second reason for cancer morbidity worldwide. Long noncoding RNA (LncRNA) HOX transcript antisense RNA (HOTAIR) has recently emerged as a promoter of metastasis in various cancer types, including GC, through the epithelial‑mesenchymal transition (EMT) process. However, the exact mechanism of HOTAIR in promoting EMT is unknown. Aberrant expression of the miR-200 family has been linked to the occurrence and development of various types of malignant tumors. This study investigates the correlation between the HOTAIR and miR-200 family gene expression patterns in GC cell lines. We investigated the miR-200 and HOTAIR due to their common molecular features in the EMT process. Methods: AGS and MKN45 cell lines were transfected with si-HOTAIR, along with a negative control. The effect of HOTAIR knockdown was also analyzed on cell viability and also on the expression of miR-200 family members, including miR-200a, -200b, and -200c, in cell lines using qRT-PCR. Statistical analysis was performed to find the potential correlation between the expression level of HOTAIR and miRs. Results: Our results showed significant increased miR-200 family expression level in transfected AGS and MKN45 GC cells (fold changes > 2; p < 0.001). Moreover, a negative correlation was observed between HOTAIR and miR-200 expression levels in GC cell lines (p < 0.05). Conclusion: Our findings showed a significant association between miR-200 family and HOTAIR expression levels in GC cell lines. Taken together, the HOTAIR-miR-200 axis seems to play a vital role in human GC, suggesting a potential therapeutic target in future GC treatment. Gene expression, Long noncoding RNA, HOTAIR, MicroRNAs 77 84 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-4573-1&slc_lang=en&sid=1 Fatemeh Bossaghzadeh Bossagh_f_1359@yahoo.com 0000-0002-9406-7251 No Department of Biology, Science and research Branch, Islamic Azad University, Tehran, Iran; Mohammadreza Hajjari Mohamad.hajari@gmail.com 0000-0002-3838-0259 Yes Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran Abdolkarim Sheikhi sheikhi@queensu.ca 0000-0002-3559-5548 No Department of Immunology, Faculty of Medicine, Dezful University of Medical Sciences, Dezful, Iran Iman Salahshourifar isalahshouri@gmail.com 0000-0001-9987-1188 No Department of Biology, Science and research Branch, Islamic Azad University, Tehran, Iran Shiva Irani shi_irani@yahoo.com 0000-0002-4202-9931 No Department of Biology, Science and research Branch, Islamic Azad University, Tehran, Iran