en Related Fields Related Fields مقاله کوتاه Short Communication <div style="direction: ltr;"><strong>Background:</strong> Methadone therapy is a major protocol in opioid addiction cases in many health care systems. Population-based studies have shown that in addicted people, the genetic profile affects their response to methadone therapy. Therefore, this study designed to examine the frequency of two SNPs of the <em>CYP2B6</em> gene (rs3745274 and rs3211371) in addicted cases in two methadone-responders and methadone non-responders groups. <strong>Methods: </strong>A total of 199 opioid-addicted individuals and 117 unaffected control subjects were genotyped for rs3745274 and rs3211371 polymorphisms of the <em>CYP2B6</em> gene using the tetra-primer amplification refractory mutation system-polymerase chain reaction. <strong>Results: </strong>Results of this study revealed the significant association of rs3745274 GG (<em>p</em> < 0.001; OR = 0.027; 95% CI = 0.14-0.49) and GT (<em>p</em> < 0.001; OR = 4.04; 95% CI = 2.26-7.21) genotypes with the risk of addiction in methadone-responders. Also, a significant association between rs3745274 GG (<em>p</em> < 0.001; OR = 0.28; 95% CI = 0.15-0.51) and GT (<em>p</em> < 0.001; OR = 5.1; 95% CI = 2.8-5.28) genotypes and addiction relapse was found in methadone non-responders. <strong>Conclusion: </strong>Based on our findings, we can conclude that rs3745274 variant of <em>CYP2B6</em> gene could serve as a potential biomarker, to evaluate the prognosis of addicted people fate under treatment with methadone.</div> Addiction, Biomarker, Methadone, Single-nucleotide polymorphism 220 225 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-4348-1&slc_lang=en&sid=1 Sara Sadat Aghabozorg Afjeh 0000-0002-6352-9310 No Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran Behzad Boshehri 0000-0001-5082-1470 No Department of Forensic Medicine and Toxicology, Urmia University of Medical Sciences, Urmia, Iran Safar Hamednia 0000-0003-3924-9826 No Department of Psychiatry, Urmia University of Medical Sciences, Urmia, Iran Asmaolhosna Amini 0000-0002-4170-0738 No Sara Medical Genetic Laboratory, Tehran, Iran Parisa Mashayekhi 0000-0002-6373-3266 No Tajrish research center Pasteur Institute of Iran,, Tehran, Iran Mir Davood Omrani 0000-0002-0673-9717 Yes Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran