en Molecular Immunology & Vaccines Molecular Immunology & Vaccines مقاله کامل Full Length/Original Article <strong>Background:</strong> Cystic echinococcosis is a zoonotic parasitic infection caused by <em>Echinococcus granulosus</em> worldwide and is associated with economic losses among livestock animals<em>. </em>EG95 is an immunogenic antigen from the <em>E. granulosus.</em> <em>Lactococcus lactis </em>has been prested as a safe vehicle for antigen delivery. The goal of this study was to design a novel <em>L. lactis</em> strain displaying EG95 as a vaccine delivery system.<strong> Methods:</strong> The <em>eg</em>95 encoding gene fragment fused to the M6 anchoring protein was cloned into the pNZ7021 vector, and<em> L. lactis</em> NZ9000 displaying recombinant EG95 was constructed. The expression of an approximately 32-kDa EG95 protein was confirmed by Western blotting and immunofluorescence analysis. The immune responses were evaluated in BALB/c mice immunized orally and subcutaneously with the live and killed recombinant <em>L. lactis</em>, respectively. <strong>Results:</strong> Total IgG level in mice immunized with heat-killed recombinant <em>L. lactis </em>(pNZ7021-<em>eg</em>95) significantly increased compared to the control group. Mucosal IgA was significantly higher in mice received live recombinant <em>L. lactis </em>(pNZ7021-<em>eg</em>95) compared to the control mice. Splenic lymphocytes from immunized mice represented the high levels of IFN-&gamma; and the low-levels of IL-4 and IL-10. <strong>Conclusion:</strong> Our results indicate that immunization with EG95-expressing <em>L. lactis </em>can induce both specific humoral and cellular immune responses in mice. Echinococcus granulosus, Lactococcus lactis, Immunization, Vaccines 284 296 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-4318-1&slc_lang=en&sid=1 Fatemeh Ebrahimzadeh febrahimzadeh2010@gmail.com 0000-0001-5295-3530 No Department of Medical Biotechnology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran Hoda Shirdast Mitra Saffari Manager) shirdast.hoda@gmail.com 0000-0002-1409-869X No Department of Medical Biotechnology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran Amirhossein Taromchi taromchi@yahoo.com 0000-0003-2268-6544 Yes Department of Medical Biotechnology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran Yeganeh Talebkhan yeganeh2005@yahoo.com 0000-0003-1988-4419 No Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran; 3Department of Medical Parasitology and Mycology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran Ali Haniloo hani@zums.ac.ir 0000-0002-3200-3981 No Department of Medical Parasitology and Mycology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran Abdolreza Esmaeilzadeh a46reza@zums.ac.ir 0000-0002-5402-3967 No Department of Immunology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran Keivan Nedaei nedaee.k@gmail.com 0000-0002-0616-3244 No Department of Medical Biotechnology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran Esmat Mirabzadeh mirabzadeh@pasteur.ac.ir 0000-0003-3636-0780 No Department of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran