en Related Fields Related Fields مقاله کامل Full Length/Original Article <span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:normal"><span sans-serif=""><b><span style="font-size:10.5pt"><span new="" roman="" times="">Background:</span></span></b><span style="font-size:10.5pt"><span new="" roman="" times=""> Differential diagnosis of <span style="color:#242021">chronic lymphoproliferative disorders</span> (CLDs) <span style="color:black">has remained</span> challenging due to the highly variable morphology features and immunophenotyping. Currently, the development of multiple-marker panel analyses by flow cytometry <span style="background:white"><span style="color:black">has opened a broad way for diagnosis of CLDs. </span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif=""><b><span style="font-size:10.5pt"><span style="background:white"><span new="" roman="" times=""><span style="color:black">Methods:</span></span></span></span></b><span style="font-size:10.5pt"><span style="background:white"><span new="" roman="" times=""><span style="color:black"> We analyzed the peripheral blood and </span></span></span></span><span style="font-size:10.5pt"><span new="" roman="" times=""><span style="color:#242021">b</span></span></span><span style="font-size:10.5pt"><span new="" roman="" times="">one marrow<span style="background:white"><span style="color:black"> samples of 131 patients with B-cell CLDs (including 91 </span></span><span style="color:#242021">chronic lymphocytic leukemia</span><span style="background:white"><span style="color:black"> (CLL), 15 atypical CLL, 14 </span></span></span></span><span style="font-size:10.5pt"><span new="" roman="" times="">mantle cell lymphoma</span></span><span style="font-size:10.5pt"><span style="background:white"><span new="" roman="" times=""><span style="color:black"> (MCL), and 11 </span></span></span></span><span style="font-size:10.5pt"><span new="" roman="" times="">CD5-/CD10-lymphoma patients) from April 2018 to April 2019, using a panel of specific markers by flow cytometry<span style="background:white"><span style="color:black">. </span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif=""><b><span style="font-size:10.5pt"><span style="background:white"><span new="" roman="" times=""><span style="color:black">Results:</span></span></span></span></b><span style="font-size:10.5pt"><span style="background:white"><span new="" roman="" times=""><span style="color:black"> Our results indicated that </span></span></span></span><span style="font-size:10.5pt"><span new="" roman="" times="">the expression pattern of CD22, CD23, FMC-7, and CD5 allowed us to accurately and differentially diagnose the B-CLL, MCL, and CD5-/CD10- lymphoma, while it was not capable of differentiating MCL from atypical CLL. W</span></span><span style="font-size:10.5pt"><span style="background:white"><span new="" roman="" times=""><span style="color:black">e, however, found that the expression patterns of CD38 and immunoglobulin light chain differed significantly between atypical B-CLL and MCL. CD38 and lambda light chain were remarkably expressed in MCL patients (92.8% and 85%, respectively) compared to the atypical CLL (1.1% and 0% respectively), with the <i>p</i> value less than 0.001 for both markers. In contrast to MCL patients, all the patients with atypical CLL, expressed kappa light chain.</span></span></span></span><span style="font-size:10.5pt"><span new="" roman="" times=""> The </span></span><span style="font-size:10.5pt"><span new="" roman="" times="">immunohistochemistry</span></span><span style="font-size:10.5pt"><span new="" roman="" times=""> method used for cyclin D1 confirmed that the flow cytometry detection of kappa and lambda light chains could provide a new approach with&nbsp;high sensitivity&nbsp;(91%) and&nbsp;moderate specificity (50%) to distinguish MCL patients from atypical B-CLL. </span></span></span></span></span><br> <b><span style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" times="">Conclusion:</span></span></span></b><span style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" times=""> Expression of CD5, CD20 (bright), CD22, FMC-7, CD38, and lambda light chain with no expression of CD23 can accurately detect MCL and differentiate it from</span></span></span> <span style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" times="">atypical B-CLL. </span></span></span></span> Chronic lymphocytic leukemia, Flow cytometry, Immunoglobulin light chain, Lymphoma 15 22 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-4246-1&slc_lang=en&sid=1 Mahdieh Mehrpouri mahdiyemehrpoori@gmail.com 0000-0001-7259-4811 No Department of Laboratory Sciences, School of Allied Medical Sciences, Alborz University of Medical Sciences, Karaj, Iran Maryam Sadat Hosseini m.hosseini1369@gmail.com 0000-0002-7482-6185 No Department of Hematology and Blood Banking, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran Leila Jafari Leila_jafari1367@yahoo.com 0000-0001-6838-2963 No Department of Hematology and Blood Banking, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran Mohammad Mosleh farahanimohammad86@gmail.com 0000-0002-9909-4611 No Department of Hematology and Blood Banking, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran Esmaeil Shahabi Satlsar esmaeilshahabi@yahoo.com 0000-0001-5259-059X Yes Department of Laboratory Sciences, School of Allied Medical Sciences, Guilan University of Medical Sciences, Guilan, Iran. Department of Flow Cytometry, Takhte Tavous Pathobiology Laboratory, Tehran, Iran