Iranian Biomedical Journal
مجله بیومدیکال ایران
IBJ
Basic Sciences
http://ibj.pasteur.ac.ir
1
admin
1028-852X
2008-823X
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10.61186/ibj
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8888
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en
jalali
1399
10
1
gregorian
2021
1
1
25
1
online
1
fulltext
en
Developing a Fluorescent Hybrid Nanobiosensor Based on Quantum Dots and Azoreductase Enzyme forMethyl Red Monitoring
Related Fields
Related Fields
مقاله کامل
Full Length/Original Article
<strong>Background:</strong> Azo dyes are the most widely used synthetic colorants in the textile, food, pharmaceutical, cosmetic, and other industries, accounting for nearly 70% of all dyestuffs consumed. Recently, much research attention has been paid to efficient monitoring of these hazardous chemicals and their related metabolites because of their potentially harmful effect on environmental issues. In contrast to the complex and expensive instrumental procedures, the detection system based on the quantum dots (QDs) with the superior optochemical properties provides a new era in the pollution sensing and prevention. <strong>Methods:</strong> We have developed a QD-enzyme hybrid system to probe methyl red (MR) in aqueous solutions using a fluorescence quenching procedure. <strong>Results: </strong>The azoreductase enzyme catalyzed the reduction of azo group in MR, which can efficiently decrease the Förster resonance energy transfer between the QDs and MR molecules. The correlation between the QDs photoluminescence recovery and MR enzymatic decolorization at the neutral phosphate buffer permitted the creation of a fluorescence quenching-based sensor. The synthesized biosensor can be used for the accurate detection of MR in a linear calibration over MR concentrations of 5-84 μM, with the limit of detection of 0.5 μM in response time of three minutes. <strong>Conclusion:</strong> Our findings revealed that this fluorometric sensor has the potential to be successfully applied for monitoring a wide linear range of MR concentration with the relative standard deviation of 4% rather than the other method.
Azoreductase, Methyl red, Quantum dots
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http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-843&slc_lang=en&sid=1
Fahimeh
Hajipour
100319475328460054252
100319475328460054252
No
Department of Biochemistry, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran
Sedigheh
Asad
100319475328460054253
100319475328460054253
No
Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran
Mohammad Ali
Amoozegar
100319475328460054254
100319475328460054254
No
Extremophiles Laboratory, Department of Microbiology, Faculty of Biology, College of Sciences, University of Tehran, Tehran, Iran
Ali Asghar
Javidparvar
100319475328460054255
100319475328460054255
No
School of Metallurgy and Materials Engineering, College of Engineering, University of Tehran, Tehran, Iran
Jialun
Tang
100319475328460054256
100319475328460054256
No
Beijing Key Laboratory of Nanophotonics and Ultrafine Optoelectronic Systems, School of Materials Science and Engineering, Beijing Institute of Technology, Beijing, China
Haizheng
Zhong
100319475328460054257
100319475328460054257
No
Beijing Key Laboratory of Nanophotonics and Ultrafine Optoelectronic Systems, School of Materials Science and Engineering, Beijing Institute of Technology, Beijing, China
Khosro
Khajeh
100319475328460054258
100319475328460054258
Yes
Department of Biochemistry, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran