en Related Fields Related Fields مقاله کامل Full Length/Original Article <strong>Background</strong>: Single nucleotide polymorphisms in 8-oxoguanine DNA glycosylase-1 (<em>OGG1</em>) gene modulates DNA repair capacity and functions as one of the first lines of protective mechanisms against 8-hydroxy-2&rsquo;-deoxyguanosine (8-OHdG) mutagenicity. <em>OGG1</em>-Cys326 gene polymorphism may decrease DNA repair function, causing oxidative stress due to higher oxidative DNA damage. The main purpose of this study was to examine the link of oxidative and genotoxic DNA damage with DNA repair <em>OGG1 </em>gene polymorphism, in charcoal workers exposed to polyaromatic hydrocarbons. <strong>Methods:</strong> Urinary 8-OHdG excretion (a biomarker of oxidative DNA damage) was determined in both exposed and control populations. Genotyping of <em>OGG1</em> DNA repair gene in the blood samples of subjects was carried out by PCR-RFLP method. <strong>Results: </strong>The 8-OHdG urinary concentration was significantly higher (<em>p</em> < 0.05) in the exposed (geometric mean 12.33 &plusmn; 3.78) than in the unexposed (geometric mean 7.36 &plusmn; 2.29) population. DNA damage, as measured by 8-OHdG and tail moment content, was found to be significantly higher in <em>OGG1</em> homozygous mutants (mt/mt; 18.81 &plusmn; 3.34; 6.04 &plusmn; 0.52) as compared to wild-type genotypes (wt/wt; 10.34 &plusmn; 2.25; 5.19 &plusmn; 2.50) and heterozygous (wt/mt) mutants (12.82 &plusmn; 2.81; 6.04 &plusmn; 0.93) in the exposed group. <strong>Conclusion:</strong> We found a significant association of <em>OGG1</em> heterozygous (<em>wt/mt</em>) and homozygous (<em>mt/mt</em>) variants with oxidative and genotoxic damage, suggesting that these polymorphisms may modulate the effects of polycyclic aromatic hydrocarbons exposure in occupational workers. 8-hydroxy-2’-deoxyguanosine, 1-hydroxypyrene, Polycyclic aromatic hydrocarbons 47 53 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-840&slc_lang=en&sid=1 Kanika Miglani 0000-0003-4104-5048 No Department of Biochemistry, Kurukshetra University Kurukshetra, Haryana 136119, India Sunil Kumar 0000-0002-7845-1341 No Department of Biotechnology, Kurukshetra University Kurukshrtra, Haryana 136119, India Anita Yadav 0000-0002-4586-2064 No Department of Biotechnology, Kurukshetra University Kurukshrtra, Haryana 136119, India Neeraj Aggarwal 0000-0003-3515-1207 No Department of Microbiology, Kurukshetra University Kurukshetra, Haryana 136119, India Ranjan Gupta 0000-0001-5684-2491 Yes Department of Biochemistry, Kurukshetra University Kurukshetra, Haryana 136119, India