en Enzymology and Protein Chemistry Enzymology and Protein Chemistry مقاله کامل Full Length/Original Article <strong>Background</strong>: This study was devoted to assessing the inhibitory potential of acetone, methanol, and ethanol extracts of <em>Acroptilon repens</em> against disease-associated enzymes, as well as their antioxidant/antibacterial activity and phytochemical composition. <strong>Methods</strong>: Comparative assessment using various antioxidant evaluation methods, including ferric reducing antioxidant power, scavenging ability on 2,2-diphenyl-1-picrylhydrazyl radical and hydrogen peroxide, and reducing power, indicated that the acetone extract presented the highest antioxidant activity, due to its highest total antioxidant content. <strong>Results</strong>: The total phenolic content and total flavonoids content of these extracts were 3.44 &plusmn; 0.32 mg GAE/g DW and 2.09 &plusmn; 0.2 mg QE/g DW, respectively. The hydrodistillation essential oil from <em>A. repens</em> was analyzed by gas chromatography-mass spectroscopy, and 17 compounds were identified. All extracts showed good inhibitory activities against disease-related enzyme acetylcholinesterase and &alpha;-amylase, with the lowest IC₅₀ for acetonic extract. Extracts of <em>A. repens</em> exhibited inhibiting activities against the Gram-positive bacteria, with the most effect of acetone extract. <strong>Conclusion</strong>: Our findings suggest <em>A. repens</em> as a promising source of natural antioxidant, antimicrobial, anti-cholinesterase and anti-amylase agents for the management of oxidative damage, and pharmaceutical, food, and cosmeceutical purposes. Acroptilon repens, Antioxidants, Phytochemicals 21 32 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-838&slc_lang=en&sid=1 Moslem Afsharnezhad 0000-0003-0669-128X No Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran S. Shirin Shahangian 0000-0001-9861-4702 Yes Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran Behnam Rasti 0000-0002-5061-3142 No Department of Microbiology, Faculty of Basic Sciences, Lahijan Branch, Islamic Azad University (IAU), Lahijan, Guilan, Iran Mohammad Faezi Ghasemi 0000-0003-2188-176X No Department of Microbiology, Faculty of Basic Sciences, Lahijan Branch, Islamic Azad University (IAU), Lahijan, Guilan, Iran