Iranian Biomedical Journal
مجله بیومدیکال ایران
IBJ
Basic Sciences
http://ibj.pasteur.ac.ir
1
admin
1028-852X
2008-823X
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10.61186/ibj
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8888
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en
jalali
1399
10
1
gregorian
2021
1
1
25
1
online
1
fulltext
other
In silico Analysis of Pasteurella multocida PlpE Protein Epitopes As Novel Subunit Vaccine Candidates
Related Fields
Related Fields
مقاله کامل
Full Length/Original Article
<strong>Background: </strong><em>Pasteurella multocida </em>is a Gram-negative, non-motile, non-spore forming, and aerobic/anaerobic cocobacillus known as the causative agent of human and animal diseases. Humans can often be affected by cat scratch or bite, which may lead to soft tissue infections and in rare cases to bacteremia and septicemia. Commercial vaccines against this agent include inactivated, live attenuated, and non-pathogenic bacteria. Current vaccines have certain disadvantages such as reactogenicity or reversion to virulence. Therefore, the aim of this study was to reach a multi-epitope vaccine candidate that could be serotype independent and covers most incident serotypes of <em>P. multocida</em>. <strong>Methods: </strong>In this study, reverse vaccinology strategy was used to identify potentially immunogenic and protective epitopes. First, multiple alignments of different sequences of Pasteurella lipoprotein E (PlpE) from various serotypes of <em>P. multocida</em> were analyzed to identify the conserved regions. Bioinformatics tools were then applied to predict and select epitopes for further studies. <strong>Results: </strong>Three different conserved immunogenic regions were selected according to the selected criteria, and their various sequential orders were evaluated structurally by <em>in silico</em> tools to find the best order. <strong>Conclusion: </strong>In searching the epitopes of PlpE to design a new vaccine candidate against pasteurellosis, we found the region 1 + region 2 + region 3 (without any linker between regions) of epitope, including the regions of PlpE protein of <em>P. multocida</em>, as the appropriate serotype independent vaccine candidate against pasteurellosis.
Pasteurella multocida, Polytope, Vaccines
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http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-837&slc_lang=other&sid=1
Saied
Mostaan
100319475328460054268
100319475328460054268
No
Molecular Biology Department, Pasteur Institute of Iran, Tehran, Iran
Abbas
Ghasemzadeh
100319475328460054269
100319475328460054269
No
Molecular Biology Department, Pasteur Institute of Iran, Tehran, Iran
Parastoo
Ehsani
100319475328460054270
100319475328460054270
No
Molecular Biology Department, Pasteur Institute of Iran, Tehran, Iran
Soroush
Sardari
100319475328460054271
100319475328460054271
No
Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
Mohammad Ali
Shokrgozar
100319475328460054272
100319475328460054272
No
National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran
Mohsen
Abolhassani
100319475328460054273
100319475328460054273
Yes
Hybridoma Lab, Department of Immunology, Pasteur Institute of Iran, Tehran, Iran
Gholamreza
Nikbakht Brujeni
100319475328460054274
100319475328460054274
No
Department of Microbiology and Immunology, University of Tehran, Iran