Iranian Biomedical Journal
مجله بیومدیکال ایران
IBJ
Basic Sciences
http://ibj.pasteur.ac.ir
1
admin
1028-852X
2008-823X
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10.61882/ibj
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8888
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en
jalali
1402
6
1
gregorian
2023
9
1
27
5
online
1
fulltext
en
Optimal Extraction and Deproteinization Method for Mannoprotein Purification from Kluyveromyces marxianus
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مقاله کامل
Full Length/Original Article
<span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:107%"><b><span style="font-size:10.5pt"><span style="line-height:107%"><span style="color:black">Background:</span></span></span></b> <span style="font-size:10.5pt"><span style="line-height:107%"><span style="color:black">Mannoproteins, mannose-glycosylated proteins, play an important role in biological processes and have various applications in industries. Several methods have been already used for the extraction of mannoproteins from yeast cell-wall.</span></span></span><span style="font-size:10.5pt"><span style="line-height:107%"><span style="color:black"> The aim of this study was to evaluate the extraction and deproteinization of mannan oligosaccharide from the </span></span></span><i><span style="font-size:10.5pt"><span style="line-height:107%"><span style="color:black">Kluyveromyces </span></span></span></i><span style="font-size:10.5pt"><span style="line-height:107%"><span style="color:black">(</span></span></span><i><span style="font-size:10.5pt"><span style="line-height:107%"><span style="color:black">K.</span></span></span></i><span style="font-size:10.5pt"><span style="line-height:107%"><span style="color:black">)<i> marxianus</i> mannoprotein.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:107%"><b><span style="font-size:10.5pt"><span style="line-height:107%"><span style="color:black">Methods: </span></span></span></b><span style="font-size:10.5pt"><span style="line-height:107%"><span style="color:black">To acquire crude mannan oligosaccharides, <i>K. marxianus </i>mannoproteins were deproteinized by the Sevage, </span></span></span><span lang="EN-GB" style="font-size:10.5pt"><span style="line-height:107%"><span style="color:black">trichloroacetic acid</span></span></span><span style="font-size:10.5pt"><span style="line-height:107%"><span style="color:black">, and </span></span></span><span lang="EN-GB" style="font-size:10.5pt"><span style="line-height:107%"><span style="color:black">hydrochloric acid</span></span></span> <span style="font-size:10.5pt"><span style="line-height:107%"><span style="color:black">(HCL) methods. Total nitrogen, crude protein content, fat, carbohydrate and ash content were measured according to the monograph prepared by the meeting of the Joint FAO/WHO Expert Committee and standard. Mannan oligosaccharide loss, percentage of deproteinization, and chemical composition of the product were assessed to check the proficiency of different methods.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:107%"><b><span style="font-size:10.5pt"><span style="line-height:107%"><span style="color:black">Results:</span></span></span></b><span style="font-size:10.5pt"><span style="line-height:107%"><span style="color:black"> Highly purified (95.4%) mannan oligosaccharide with the highest deproteinization (97.33 ± 0.4%) and mannan oligosaccharide loss (25.1 ± 0.6%) were obtained following HCl method.</span></span></span></span></span><br>
<b><span style="font-size:10.5pt"><span style="line-height:107%"><span style="color:black">Conclusion:</span></span></span></b><span style="font-size:10.5pt"><span style="line-height:107%"><span style="color:black"> HCl, was the most appropriate deproteinization method for the removal of impurities. This preliminary data will support future studies to design scale-up procedures. </span></span></span></span>
Kluyveromyces marxianus, Mannan oligosaccharides, Downstream extraction process
320
325
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-2110-1&slc_lang=en&sid=1
Ashraf
Hajhosseini