Iranian Biomedical Journal
مجله بیومدیکال ایران
IBJ
Basic Sciences
http://ibj.pasteur.ac.ir
1
admin
1028-852X
2008-823X
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10.61186/ibj
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8888
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en
jalali
1399
12
1
gregorian
2021
3
1
25
2
online
1
fulltext
en
Anti-Proliferative Properties, Biocompatibility, and Chemical Composition of Different Extracts of Plantago major Medicinal Plant
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مقاله کامل
Full Length/Original Article
<div id="_com_1" uage="JavaScript"><strong>Background:</strong> To study the anticancer activity of <em>Plantago major</em>, we assessed the effect of ethanolic, methanolic and acetonic extracts of this plant on HCT-116, SW-480, and HEK-293 cell lines as control. <strong>Methods:</strong> The cytotoxic activity, biocompatibility, and toxicity were evaluated by MTT assay, hemolysis, and <em>Artemia salina</em>-LD<sub>50</sub> (on mice) tests, respectively. The analysis of the extracts was performed by GC-MS analysis. <strong>Results:</strong> The results showed that all the extracts had the most antiproliferative properties on the HCT-116 cell line. The <em>P. major</em> root extract was more effective than the aerial parts, and IC<sub>50</sub> values for ethanolic, methanolic and acetonic root extracts were 405.59, 470.16, and 82.26 µg/mL, respectively on HCT-116 cell line at 72 h. Hemolysis degree of the ethanolic extract of aerial and root parts were approximately 1% at 400 μg/mL.. Using the ethanolic extracts, the <em>Artemia</em> survived every concentration, and no toxicity was observed. One week after the oral administration of different parts of <em>P. major </em>extracts, none of the mice died, even those were administered 2000 mg/kg. The results of GC/MS analysis showed that <em>P. major</em> extracts contain potential anticancer compounds, such as stearic acid (8.61%) in aerial parts of methanolic extract and 1,2- Benzenedicarboxylic acid, mono(2-ethylhexyl)ester (88.07% and 40.63%) in aerial and root parts of acetonic extract of <em>P. major</em>. <strong>Conclusions:</strong> Our findings suggest that the <em>P. major</em> is a source of potential compounds with antiproliferative properties.</div>
Gas chromatography-mass spectrometry, HCT-116 cells, Hemolysis, Lethal dose 50
106
116
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-3887-1&slc_lang=en&sid=1
Samaneh
Rahamouz-Haghighi
samaneh.rahamooz@znu.ac.ir
100319475328460054238
100319475328460054238
No
Department of Plant Production and Genetics, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
Khadijeh
Bagheri
bagheri.khadijeh@znu.ac.ir
100319475328460054239
100319475328460054239
No
Department of Plant Production and Genetics, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
Hossein
Danafar
danafar@zums.ac.ir
100319475328460054240
100319475328460054240
No
Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences 45139-56184, Zanjan; Iran
Ali
Sharafi
sharafi.a@gmail.com
100319475328460054241
100319475328460054241
Yes
Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences 45139-56184, Zanjan; Iran