en Related Fields Related Fields مقاله کامل Full Length/Original Article <div id="_com_1" uage="JavaScript"><strong>Background:</strong> To study the anticancer activity of <em>Plantago major</em>, we assessed the effect of ethanolic, methanolic and acetonic extracts of this plant on HCT-116, SW-480, and HEK-293 cell lines as control. <strong>Methods:</strong> The cytotoxic activity, biocompatibility, and toxicity were evaluated by MTT assay, hemolysis, and <em>Artemia salina</em>-LD₅₀ (on mice) tests, respectively. The analysis of the extracts was performed by GC-MS analysis. <strong>Results:</strong> The results showed that all the extracts had the most antiproliferative properties on the HCT-116 cell line. The <em>P. major</em> root extract &nbsp;was more effective than the aerial parts, and IC₅₀ values for ethanolic, methanolic and acetonic root extracts were 405.59, 470.16, and 82.26 &micro;g/mL, respectively on HCT-116 cell line at 72 h. Hemolysis degree of the ethanolic extract of &nbsp;aerial and root parts were approximately 1% at 400 &mu;g/mL.. Using the ethanolic extracts, the <em>Artemia</em> survived every concentration, and no toxicity was observed. One week after the oral administration of different parts of <em>P. major </em>extracts, none of the mice died, even those were administered 2000 mg/kg. The results of GC/MS analysis showed that <em>P. major</em> extracts contain potential anticancer compounds, such as stearic acid (8.61%) in aerial parts of methanolic extract and 1,2- Benzenedicarboxylic acid, mono(2-ethylhexyl)ester (88.07% and 40.63%) in aerial and root parts of acetonic extract of <em>P. major</em>. <strong>Conclusions:</strong> Our findings suggest that the <em>P. major</em> is a source of potential compounds with antiproliferative properties.</div> Gas chromatography-mass spectrometry, HCT-116 cells, Hemolysis, Lethal dose 50 106 116 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-3887-1&slc_lang=en&sid=1 Samaneh Rahamouz-Haghighi samaneh.rahamooz@znu.ac.ir 0000-0002-6012-1424 No Department of Plant Production and Genetics, Faculty of Agriculture, University of Zanjan, Zanjan, Iran Khadijeh Bagheri bagheri.khadijeh@znu.ac.ir 0000-0002-1434-8062 No Department of Plant Production and Genetics, Faculty of Agriculture, University of Zanjan, Zanjan, Iran Hossein Danafar danafar@zums.ac.ir 0000-0001-8956-7895 No Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences 45139-56184, Zanjan; Iran Ali Sharafi sharafi.a@gmail.com 0000-0002-6012-1424 Yes Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences 45139-56184, Zanjan; Iran