en Related Fields Related Fields مقاله کامل Full Length/Original Article <strong>Background:</strong> Our previous findings indicated that carvacrol and thymol alleviate cognitive impairments caused by A&beta; in rodent models of Alzheimer&#39;s disease (AD). In this study, the neuroprotective effects of carvacrol and thymol against A&beta;_25-35-induced cytotoxicity were evaluated, and the potential mechanisms were determined. <strong>Methods:</strong> PC12 cells were pretreated with A&beta;_25-35 for 2 h, followed by incubation with carvacrol or thymol for additional 48 h. Cell viability was measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. A flurospectrophotometer was employed to observe the intracellular reactive oxygen species (ROS) production. Protein kinase C (PKC) activity was analyzed using ELISA. <strong>Results:</strong> Our results indicated that carvacrol and thymol could significantly protect PC12 cells against A&beta;_25-35-induced cytotoxicity. Furthermore, A&beta;_25-35could induce intracellular ROS production, while carvacrol and thymol could reverse this effect. Moreover, our findings showed that carvacrol and thymol elevate PKC activity similar to Bryostatin-1, as a PKC activator. <strong>Conclusion: </strong>This study provided the evidence regarding the protective effects of carvacrol and thymol against A&beta;_25&ndash;35-induced cytotoxicity in PC12 cells. The results suggested that the neuroprotective effects of these compounds against&nbsp;A&beta;_25-35 might be through attenuating oxidative damage and increasing the activity of PKC as a memory-related&nbsp;protein. Thus, carvacrol and thymol were found to have therapeutic potential in preventing or modulating AD. Alzheimer’s disease, Carvacrol, Thymol, Reactive oxygen species, Protein kinase C 243 250 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-812&slc_lang=en&sid=1 Zahra Azizi 0000-0001-5094-1333 Yes Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran Mona Salimi 0000-0002-3997-7009 No Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran Amir Amanzadeh 0000-0003-0825-6951 No Department of Cell Bank, Pasteur Institute of Iran, Tehran, Iran Nahid Majelssi 0000-0002-7941-944X No Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran Nasser Naghdi 0000-0002-3315-372X No Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran