Iranian Biomedical Journal
مجله بیومدیکال ایران
IBJ
Basic Sciences
http://ibj.pasteur.ac.ir
1
admin
1028-852X
2008-823X
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10.61882/ibj
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8888
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en
jalali
1399
2
1
gregorian
2020
5
1
24
3
online
1
fulltext
other
Visualization of Leishmania tropica Infection in BALB/c Mice by Bioluminescence Imaging
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مقاله کامل
Full Length/Original Article
<strong>Background:</strong> <em>Leishmania tropica</em> is the cause of more than one form of leishmaniasis and lacks a known reservoir animal. This study compares the potential infectivity of recombinant and wild-type <em>L. tropica</em> in BALB/c mice. <strong>Methods:</strong> The potential infectivity of recombinant<em> L. tropica</em><sup>EGFP</sup> or <em>L. tropica</em><sup>EGFP-LUC</sup> by two different, the subcutaneous and intradermal, routes was compared using a range of classical detection methods and bioluminescence imaging (BLI). <strong>Results:</strong> In addition to the results obtained from classical diagnostic approaches, the BLI signals were detected in footpads and ears of <em>L. tropica-</em>infected animals. The BLI revealed that a bioluminescence signal can be observed at the inoculation site. The stability of the BLI remained constant in the footpad, but the signal was detectable for only three months in the pinna due to the decline in infection over time. <strong>Conclusion:</strong> The presented data are a precise verification of the assumption that BALB/c mice could be used as an experimental model for <em>L. tropica </em>infectivity.
BALB/c mice, Green fluorescent protein, Leishmania tropica, Luciferase
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172
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-805&slc_lang=other&sid=1
Mahdieh
Eskandar