en Tissue Engineering and Cell Biology Tissue Engineering and Cell Biology مقاله کامل Full Length/Original Article <strong>Background</strong>: Self-assembling peptides (SApeptides) have growing applications in tissue engineering and regenerative medicine. The application of SApeptide-based hydrogels depends strongly on their viscoelastic properties. Optimizing the properties is of importance in tuning the characteristics of the hydrogels for a variety of applications. <strong>Methods</strong>: In this study, we employed statistical modeling, conducted with the response surface methodology (RSM) and particle tracking microrheology, to investigate the effects of self-assembling SPG-178 peptide and added NaCl salt concentrations and milieu type (deionized water or blood serum) on the viscoelastic properties of SPG-178 hydrogels. A central composite&nbsp;RSM model was employed for finding the optimum value of the parameters to achieve the highest storage modulus and the lowest tan &delta;. <strong>Results:</strong> Viscoelastic properties of each sample, including storage modulus, loss modulus, and tan &delta;, were determined. Storage modulus and tan &delta; were modeled, accounting for the impact of the SPG-178 peptide and NaCl concentrations and milieu type on the viscoelastic properties. It was found that the SPG-178 hydrogel storage modulus was positively influenced by the SPG-178 peptide concentration and the serum. <strong>Conclusion:</strong> A combination of microrheology and RSM is a useful test method for statistical modeling and analysis of rheological behavior of solid-like gels, which could be applied in various biomedical applications such as hemostasis. Hydrogels, Regenerative medicine, Rheology, Tissue engineering, SPG-178 110 118 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-802&slc_lang=en&sid=1 Mansooreh-Sadat Seyedkarimi 0000-0002-5523-8927 Yes Polymer and Color Engineering Department, Amirkabir University of Technology, Tehran, Iran Hamid Mirzadeh 0000-0002-9821-1759 No Polymer and Color Engineering Department, Amirkabir University of Technology, Tehran, Iran Aliasghar Mohammadi 0000-0002-7367-225X No Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran Shadab Bagheri-Khoulenjani 0000-0002-0478-0679 No Polymer and Color Engineering Department, Amirkabir University of Technology, Tehran, Iran