Iranian Biomedical Journal
مجله بیومدیکال ایران
IBJ
Basic Sciences
http://ibj.pasteur.ac.ir
1
admin
1028-852X
2008-823X
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10.61186/ibj
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8888
-
en
jalali
1398
12
1
gregorian
2020
3
1
24
2
online
1
fulltext
en
Gene Expression of Promyelocytic Leukemia Proteins and IFN-γ Is Reduced in Rotavirus-Infected Children
Molecular Microbiology
Molecular Microbiology
مقاله کامل
Full Length/Original Article
<strong>Background:</strong> Rotavirus infection is one of the most common gastroenteritis in the world, and a million cases are registered to enter hospital every year. Promyelocytic leukemia proteins (PMLs) are IFN-up-regulated proteins, and one of their critical functions is working as antiviral proteins. Recently, promyelocytic leukemia isoform II (PML-II) has been depicted as an isoform responsible for the antiviral function. <strong>Methods: </strong>Rotavirus prevalence determination was achieved by PCR and Rapid Adeno/Rota Virus test, while the relative expression assay was carried out by real-time PCR technique<strong>. </strong>Blood and stool samples were collected from 34 children under five years admitted to the hospital with acute gastroenteritis showing signs of dehydration. RNA samples were extracted from blood specimens and converted to cDNA to be used in gene expression analysis of PML, PML-II, and IFN-γ in rotavirus positive or negative samples. <strong>Results:</strong> Rapid Adeno/Rota Virus Antigen Combo Test and PCR assay could detect the virus in stool samples in 45% and 17.6% of cases, respectively. PML in positive samples decreased to 10<sup>4</sup>fold less than the level in negative ones. The same trend was noticed in the level of IFN-γ and PML-II expression as their expression reduced to 10<sup>4</sup> or 13fold in rotavirus-infected samples compared to the control, respectively. <strong>Conclusion:</strong> Altogether, our data showed that the gene expression of PML, PML-II, and type II IFN considerably diminished in rotavirus-infected samples compared to the negative control.
Gene expression, IFN-γ, PML, PML-II, Rotavirus
128
135
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-799&slc_lang=en&sid=1
Ahmed Jasim
Mohammed
100319475328460054548
100319475328460054548
No
Department of Biology, College of Science, University of Basra, Basra, Iraq
Zeenah Weheed
Atwan
100319475328460054549
100319475328460054549
Yes
Department of Biology, College of Science, University of Basra, Basra, Iraq
Aida
Abdul-Kareem
100319475328460054550
100319475328460054550
No
Department of Biology, College of Science, University of Basra, Basra, Iraq