en Related Fields Related Fields مقاله کامل Full Length/Original Article <strong>Background: </strong>Ascorbic acid, known as vitamin C, has been used in combination with a number of cytotoxic agents <em>in vitro</em> and <em>in vivo</em> with contradictory results on its effectiveness. It is believed that vitamin C can sensitize different cancer cells to common therapy strategies such as chemotherapy and radiotherapy. During current research, the combination effect of vitamin C with cisplatin was evaluated against gastric cancer cells. <strong>Methods: </strong>MTT-based proliferation assay, combination index method, and flow cytometry technique were employed for the assessment of cell cycle and determination of apoptosis/necrosis on the AGS cell line. <strong>Results:</strong> Co-treatment of gastric cancer cells with vitamin C in its IC₅₀ dose in addition to cisplatin in both IC₅₀(10 &micro;g/ml) and five times less&nbsp;(2 &micro;g/ml) doses could increase the cytotoxicity effect of cisplatin in a synergistic manner. Moreover, the pointed co-treatment approach could induce the cell count in sub-G0 phase while reducing it in the G0/G1, G2/M, and S phases. Further findings showed that the combined dose of vitamin C and cisplatin could increase the percentage of apoptotic and necrotic cells in comparison with a single dose of cisplatin. <strong>Conclusion: </strong>This study introduces a possible approach for the treatment of gastric cancer with more potency and less amount of administered cisplatin to induce toxicity.&nbsp; Ascorbic acid, Cisplatin, Neoplasms 119 127 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-797&slc_lang=en&sid=1 Ghazaleh Ghavami 0000-0003-1251-7712 No Drug Design and Bioinformatics Unit, Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran Soroush Sardari 0000-0003-2080-7402 Yes Drug Design and Bioinformatics Unit, Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran