other Related Fields Related Fields مقاله کامل Full Length/Original Article <strong>Background:</strong> Breast cancer is one of the most prevalent cancers among women. Common cancer treatment methods are not effective enough, and there is a need for a more efficient treatment procedure. Cancer vaccine is a novel immunotherapy method that stimulates humoral and/or cellular immunity against cancer. Placenta-specific protein 1 (PLAC1) is a cancer/testis antigen, prevalent in breast cancer and rarely found in normal tissues. FliC, as a bacterial adjuvant, when fused to PLAC1 can elicit humoral and cellular responses. Therefore, PLAC1-fliC is a chimeric protein, which can be considered a suitable candidate against breast cancer. <strong>Methods: </strong>ProtParam was used to evaluate the physicochemical properties of PLAC1-fliC. Second structures were determined using the GOR V server. PLAC1-fliC 3D structure was modeled by Phyre2, and it was refined using GalaxyWEB. The refined model was submitted to RAMPAGE, PROCHECK, and ProSA-web for validation. Antigenicity and allergenicity of the construct were predicted by ANTIGENpro, VaxiJen, AllergenFP, and SDAP databases. Then MHC-I- and MHC-II-binding epitopes of PLAC1-fliC were forecasted by NetMHC 4.0 and NetMHCII 2.3 Servers. Finally, Ellipro and CTLpred were employed to predict B-cell and cytotoxic T lymphocyte epitopes. <strong>Results: </strong>The construct was evaluated as a stable fusion protein, which could be antigenic and could stimulate B and T cells against breast cancer. <strong>Conclusion: </strong>PLAC1-fliC, as a cancer vaccine candidate, might be suitable and specific for breast cancer, which could evoke humoral and cellular immunity against this type of tumor.&nbsp; Breast cancer, PLAC1, Cancer vaccines, Bioinformatics 173 182 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-796&slc_lang=other&sid=1 Mortaza Taheri-Anganeh 0000-0002-9659-7491 No Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran Ahmad Amiri 0000-0003-2752-0105 No Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran Ahmad Movahedpour No Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran Seyyed Hossein Khatami No Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran Younes Ghasemi 0000-0003-4172-0672 No Department of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran Amir Savardashtaki 0000-0003-1700-9426 No Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran Zohreh Mostafavi-Pour 0000-0002-3779-177X Yes Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran