en Tissue Engineering and Cell Biology Tissue Engineering and Cell Biology مقاله کامل Full Length/Original Article <strong>Background: </strong>The recent improvements in wound healing have led to new strategies in regenerative medicine. Burn wound healing is an important issue in skin regeneration and has multiple indications for stem cell therapy. Hair follicle stem cells (HFSCs) are a highly promising source of stem cells for healing use, as these cells are accessible, active and pluripotent adult stem cells. <strong>Methods</strong>: HFSCs of the rat whisker were isolated, cultured, and labeled with DiI. Flow cytometry method was used to detect special markers of HFSCs. Deep partial-thickness burn wound was created, and labeled HFSCs were injected around the wound bed. Wound closure was recorded via digital photographs. The inflicted rats were sacrificed at 3, 7, or 14 days post burn and used for subsequent histological and tensiometry analysis. <strong>Results:</strong> Our results indicated that HFSCs were positive for Nestin and CD34 markers, but negative for Kr15. Morphological and histological photographs revealed that wound closure rate was accelerated in stem cell-treated group compared with other groups. In addition, faster re-epithelialization and collagen deposition were observed. The immunohistochemical analysis suggested that CD31 expression and vascular density enhanced in the stem cell-treated group. Further, tissue tensile strength increased in HFSCs-treated rats in comparison to the control group. <strong>Conclusion:</strong> The present study demonstrates that HFSC could accelerate burn wound healing as well as tensile strength in rats. Burn wound healing, Epithelization, Tensile strength, Wound healing 99 109 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-789&slc_lang=en&sid=1 Azar Babakhani 0000-0001-5787-2967 No Department of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran Malihe Nobakht 0000-0003-2372-2578 Yes Department of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran Hamidreza Pazoki Torodi 0000-0001-7868-456X No Physiology Research Center, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran Mostafa Dahmardehei 0000-0003-4079-9956 No Zahedan Medical University, Zahedan, Iran Paria Hashemi 0000-0003-2813-0803 No Physiology Research Center, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran Javad Mohajer Ansari 0000-0002-6316-6716 No epartment of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran Parisa Ramhormozi 0000-0001-7366-4519 No Department of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran Abazar Yari 0000-0002-4680-9169 No Department of Anatomy, Faculty of Medicine, Alborz University of Medical Sciences, Karaj, Iran Fatemeh Heidari 0000-0003-4751-8870 No Department of Anatomy, Faculty of Medicine, Qom University of Medical Sciences, Qom, Iran