Iranian Biomedical Journal
مجله بیومدیکال ایران
IBJ
Basic Sciences
http://ibj.pasteur.ac.ir
1
admin
1028-852X
2008-823X
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10.61186/ibj
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8888
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en
jalali
1398
6
1
gregorian
2019
9
1
23
5
online
1
fulltext
other
The Lentiviral Vector Pseudotyped by Modified Rabies Glycoprotein Does Not Cause Reactive Gliosis and Neurodegeneration in Rat Hippocampus
Related Fields
Related Fields
مقاله کامل
Full Length/Original Article
<strong>Background:</strong> A human immunodeficiency virus type 1 (HIV-1)-based lentiviral vector (LV) pseudotyped by a variant of rabies envelope glycoprotein, FUG-B2, has previously been prepared and used in transfection of hippocampal CA1 (<a href="https://en.wikipedia.org/wiki/Hippocampus_anatomy#Basic_Hippocampal_Circuit" title="Hippocampus anatomy">"Cornu Ammonis" area 1</a>) neurons. This study aimed to verify reactive gliosis and neuronal damage after injection of the vector into the rat hippocampus. <strong>Methods:</strong> HEK 293T cells were transfected with transfer (fck-Jaws-GFP-ER2), envelope (FUG-B2), and packaging (pMDLg/pRRE, pRSV-Rev) plasmids, and the vector was injected into CA1 of the rat hippocampus. After one week, transduction efficiency, and the number of neuronal and astroglial cells were determined in CA1 and CA3 by double staining of the brain slices. <strong>Results:</strong> Hippocampal cells were successfully transfected as 92.7% of CA1 and 95.8% of CA3 neuronal cells expressed GFP. The frequency of neuronal and astroglial cells in CA1 and CA3 of the vector-injected rats remained unchanged compared to those in the control and the saline-injected rats. Furthermore, no morphological change was found in hippocampal astrocytes and neuronal cells.<strong> Conclusion: </strong>The HIV-1-based LV pseudotyped by FUG-B2 is safe and does not cause neuroinflammation and neuronal loss once directly delivered into the rat hippocampus.
Astrocytes, CA3 region, Rat Rabies virus glycoprotein
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http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-776&slc_lang=other&sid=1
Mostafa
Farzaneh
100319475328460054694
100319475328460054694
No
Department of Pharmacology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Mohammad
Sayyah
100319475328460054695
100319475328460054695
Yes
Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran.
Hamid Reza
Eshraghi
100319475328460054696
100319475328460054696
No
Department of Pharmacology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Negar
Panahi
100319475328460054697
100319475328460054697
No
Department of Pharmacology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Hadi
Mirzapourdelavar
100319475328460054698
100319475328460054698
No
Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran.
Hamid
Gholami Pourbadie
100319475328460054699
100319475328460054699
No
Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran.