other Related Fields Related Fields مقاله کامل Full Length/Original Article <strong>Background:</strong> A human immunodeficiency virus type 1 (HIV-1)-based lentiviral vector (LV) pseudotyped by a variant of rabies envelope glycoprotein, FUG-B2, has previously been prepared and used in transfection of hippocampal CA1 (<a href="https://en.wikipedia.org/wiki/Hippocampus_anatomy#Basic_Hippocampal_Circuit" title="Hippocampus anatomy">&quot;Cornu Ammonis&quot; area 1</a>) neurons. This study aimed to verify reactive gliosis and neuronal damage after injection of the vector into the rat hippocampus. <strong>Methods:</strong> HEK 293T cells were transfected with transfer (fck-Jaws-GFP-ER2), envelope (FUG-B2), and packaging (pMDLg/pRRE, pRSV-Rev) plasmids, and the vector was injected into CA1 of the rat hippocampus. After one week, transduction efficiency, and the number of neuronal and astroglial cells were determined in CA1 and CA3 by double staining of the brain slices. <strong>Results:</strong> Hippocampal cells were successfully transfected as 92.7% of CA1 and 95.8% of CA3 neuronal cells expressed GFP. The frequency of neuronal and astroglial cells in CA1 and CA3 of the vector-injected rats remained unchanged compared to those in the control and the saline-injected rats. Furthermore, no morphological change was found in hippocampal astrocytes and neuronal cells.<strong> Conclusion: </strong>The HIV-1-based LV pseudotyped by FUG-B2 is safe&nbsp;and does not cause neuroinflammation and neuronal loss once directly delivered into the rat hippocampus.&nbsp; Astrocytes, CA3 region, Rat Rabies virus glycoprotein 324 329 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-776&slc_lang=other&sid=1 Mostafa Farzaneh 0000-0002-8515-0942 No Department of Pharmacology, Science and Research Branch, Islamic Azad University, Tehran, Iran. Mohammad Sayyah 0000-0003-0603-2444 Yes Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran. Hamid Reza Eshraghi No Department of Pharmacology, Science and Research Branch, Islamic Azad University, Tehran, Iran. Negar Panahi No Department of Pharmacology, Science and Research Branch, Islamic Azad University, Tehran, Iran. Hadi Mirzapourdelavar No Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran. Hamid Gholami Pourbadie 0000-0002-7634-7428 No Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran.