Iranian Biomedical Journal
مجله بیومدیکال ایران
IBJ
Basic Sciences
http://ibj.pasteur.ac.ir
1
admin
1028-852X
2008-823X
-
10.61186/ibj
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8888
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en
jalali
1398
2
1
gregorian
2019
5
1
23
3
online
1
fulltext
en
Effects of Environmental Conditions on High-Yield Magnetosome Production by Magnetospirillum gryphiswaldense MSR-1
Related Fields
Related Fields
مقاله کامل
Full Length/Original Article
<strong>Background: </strong><strong> </strong>Magnetotactic bacteria are a heterogeneous group of Gram-negative prokaryote cells that produce linear chains of magnetic particles called magnetosomes, intracellular organelles composed of magnetic iron particles. Many important applications have been defined for magnetic nanoparticles in biotechnology, such as cell separation applications and acting as carriers of enzymes, antibodies or anti-cancer drugs. Since the bacterial growth is difficult and the yield of magnetosome production is low, the application of magnetosome has not been developed on a commercial scale. <strong>Methods:</strong> <em>Magnetospirillum gryphiswaldense</em> strain MSR-1 was used in a modified current culture medium supplemented by different concentrations of oxygen, iron, carbon, and nitrogen, to increase the yield of magnetosomes. <strong>Results:</strong> Our improved MSR-1 culture medium increased magnetosome yield, magnetosome number per bacterial cell, magnetic response, and bacterial cell growth yield significantly. The yield of magnetosome increased approximately four times. The optimized culture medium containing 25 mM of Na-pyruvate, 40 mM of NaNO3, 200 µM of ferrous sulfate, and 5-10 ppm of dissolved oxygen (DO) resulted in 186.67 mg of magnetosome per liter of culture medium. The iron uptake increased significantly, and the magnetic response of the bacteria to the magnetic field was higher than threefold as compared to the previously reported procedures. <strong>Conclusion: </strong>This technique not only decreases the cultivation time but also reduces the production cost. In this modified method, the iron and DO are the major factors affecting the production of magnetosome by <em>M. gryphiswaldense </em>strain MSR-1. However, refining this technique will enable a further yield of magnetosome and cell density.
Conditioned culture medium, Magnetosomes, Magnetospirillum gryphiswaldense MSR-1
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219
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-773&slc_lang=en&sid=1
Leila
Hatami-Giklou Jajan
100319475328460054853
100319475328460054853
No
Department of Research and Development, Research and Production Complex, Pasteur Institute of Iran, Karaj, Iran
Seyed Nezamedin
Hosseini
100319475328460054854
100319475328460054854
No
Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran
Masoud
Ghorbani
100319475328460054855
100319475328460054855
No
Hybridoma Lab., Department of immunology, Pasteur Institute of Iran, Tehran, Iran
Seyed Fazlollah
Mousavi
100319475328460054856
100319475328460054856
No
Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran
Behzad
Ghareyazie
100319475328460054857
100319475328460054857
No
Agriculture Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran;
Mohsen
Abolhassani
100319475328460054858
100319475328460054858
Yes
Hybridoma Lab., Department of immunology, Pasteur Institute of Iran, Tehran, Iran