en Related Fields Related Fields مقاله کوتاه Short Communication <strong>Background: </strong><em>Acinetobacter baumannii </em>is an important pathogen in health care and is responsible for severe nosocomial and community-acquired pneumonia. To design novel therapeutic agents, a mouse model for <em>A. baumannii </em>pneumonia is essential. <strong>Methods:</strong> We described a mouse model of <em>A. baumannii </em>using clinical and 19606R standard strains for developing a quantitative real-time PCR (qRT-PCR) for rapid identification of <em>A. baumannii</em> infection from lung tissues of BALB/c mice. <strong>Results:</strong> To infect the mice, three doses of bacteria (0.5 &times; 10⁸, 1 &times; 10⁸, and 1.5 &times; 10⁸ cfu/ml) were used. Lung tissues were cultured and compared with <em>ompA </em>gene. Clinical isolates had better positive results at day three with the highest dose than 19606 strain either in culture (4 versus 3) or in qRT-PCR (5 versus 4). However, qRT-PCR detection was 100%, the specificity was 70%, and the positive predictive value was 27%. <strong>Conclusion:</strong> The qRT-PCR detection of <em>A. baumannii </em>in the BALB/c mice model has a higher sensitivity than the culture method.&nbsp; Acinetobacter baumanii, OmpA, qRT-PCR Pneumonia, BALB/c mice 159 164 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-770&slc_lang=en&sid=1 Niloofar Hassannejad 0000-0002-7930-807X No Dept. of Cell Biology, Science and Research branch, Islamic Azad University, Tehran, Iran Abbas Bahador 0000-0001-5308-7208 No Dept. of Microbiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran Nasim Hayati Rudbari 0000-0002-5942-5770 No Dept. of Cell Biology, Science and Research branch, Islamic Azad University, Tehran, Iran Mohammad Hossein Modarressi 0000-0002-8203-5674 No Dept. of Medical Genetics, Tehran University of Medical Sciences, Tehran, Iran Kazem Parivar 0000-0002-7235-2852 Yes Dept. of Cell Biology, Science and Research branch, Islamic Azad University, Tehran, Iran