en Cancer Biology Cancer Biology مقاله کامل Full Length/Original Article <strong>Background: </strong>Prostate cancer (Pca) is a heterogeneous disease, and current treatments are not based on molecular stratification. Poly(adenosine diphosphate [ADP]-ribose) polymerase (PARP) inhibitors have recently been found to be remarkably toxic to cells with defects in homologous recombination, particularly cells with BRCA-mutated backgrounds. Therefore, this preliminary study was designed to evaluate whether PTEN expression status could have an impact on the sensitivity of invasive Pca cells to the PARP inhibitor, AZD2461. <strong>Methods:</strong> MTT viability test, Annexin V‐FITC/propidium iodide double staining, and caspase3 activity assay were used to evaluate the apoptosis and relative expression of PTEN and VEGF in PC-3 and DU145 cell lines using real-time PCR. <strong>Results:</strong> MTT results showed that the inhibitory effects of AZD2461 were higher in PC-3 than DU145 cells (with IC₅₀ of 36.48 and 59.03 &micro;M at 48 hours of treatment, respectively). Flow cytometric analysis also showed the same results. When exposed to 40 &micro;M of AZD2461, PC-3 (38.8%) and DU145 (28%) cells underwent apoptosis (<em>p</em> < 0.05). Treatment of cells by AZD2461 also caused a significant increase in apoptosis through caspase3 activation in both cell lines. VEGF mRNA levels in PC-3 cells significantly decreased compared to adjusted untreated cells (<em>p</em> < 0.05) in all measured times while displaying different alteration patterns in DU145 cells (<em>p</em> < 0.05). <strong>Conclusion:</strong> AZD2461 suppresses the growth of prostate tumor cells since AZD2461 monotherapy could prove to be efficacious, especially against cells not expressing PTEN besides activating the possible apoptosis-independent cell death pathways. AZD2461, DNA repair, PARP, Prostate neoplasm 312 323 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-764&slc_lang=en&sid=1 Saman Sargazi 0000-0002-2255-5977 No International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran, Ramin Saravani 0000-0003-1941-3617 Yes Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran Javad Zavar Reza 0000-0003-4065-0772 No Biotechnology Research Center, International Campus, Shahid Sadoughi University of Medical Science, Yazd, Iran, Hossein Zarei Jaliani 0000-0002-6324-3785 No Protein Engineering Laboratory, Department of Medical Genetics, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Hamidreza Galavi 0000-0003-0650-2741 No Cellular and Molecular Research Center of Zahedan University of Medical Sciences, Zahedan, Iran, Mahdiyeh Moudi 0000-0002-4502-6015 No Genetics of Non-Communicable Disease Research Center of Zahedan University of Medical Sciences, Zahedan, Iran Najmeh Alsadat Abtahi 0000-0001-9436-2982 No International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran