other Related Fields Related Fields مقاله کامل Full Length/Original Article <strong>Background: </strong>Two of the Wnt signaling pathway target genes, tumor necrosis factor receptor family member (TROY) and leucine-rich G-protein coupled receptor (LGR5), are involved in the generation and maintenance of gastrointestinal epithelium. A negative modulatory role has recently been assigned to TROY, in this pathway. Here, we have examined their simultaneous expression in gastric carcinogenesis. <strong>Methods: </strong>Tumor and paired adjacent tissues of intestinal-type gastric cancer (GC) patients (n = 30) were evaluated for LGR5 and TROY expression by immunohistochemistry. The combination of the percentage of positively&shy; stained cells and the intensity of staining was defined as the composite score and compared between groups. The obtained findings were re-evaluated in a mouse model. <strong>Results: </strong>TROY expression in the tumor tissue was significantly lower than that of the adjacent tissue (2.5 &plusmn; 0.9 vs. 3.3 &plusmn; 0.9, <em>p</em> = 0.004), which was coincident with higher LGR5 expression (3.6 &plusmn; 1.1 vs. &nbsp;2.7 &plusmn; 0.9, <em>p</em> = 0.001). This observation was prominent at stages II/III of GC, leading to a statistically significant mean difference of expression between these two molecules (<em>p</em> = 0.005). In the <em>H. pylori</em> infected-mouse model, this inverse expression was observed in transition from early (8-16 w) to late (26-50 w) time points, post treatment (<em>p</em> = 0.002). <strong>Conclusion: </strong>Our data demonstrates an inverse trend between TROY down-regulation and LGR5 up-regulation in GC tumors, as well as in response to <em>H. pylori</em> infection in mice. These findings support a potential negative modulatory role for TROY on LGR5 expression.&nbsp; Helicobacter pylori, Wnt signaling pathway, Stem Cells, Humans, Mice 107 120 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-760&slc_lang=other&sid=1 Samaneh Saberi No HPGC Research Group, Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran Abbas Piryaei 0000-0002-3182-6214 No Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Esmat Mirabzadeh 0000-0003-3636-0780 No Department of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran Maryam Esmaeili No HPGC Research Group, Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran Toktam Karimi No HPGC Research Group, Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran Sara Momtaz No HPGC Research Group, Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran Afshin Abdirad No Department of Pathology, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran Niloofar Sodeifi No Department of Andrology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran Mohammad Ali Mohagheghi No Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran Hossein Baharvand No Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran Marjan Mohammadi 0000-0002-5585-4519 Yes HPGC Research Group, Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran